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Production of Alkaline Protease by Bacillus altitudinis GVC11 using Castor Husk in Solid-State Fermentation

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Abstract

Castor (Ricinus communis L.) is an important oil seed crop having its main cultivated area in India, China, and Brazil in dry land farming. Castor husk is generated as waste in castor oil production. Use of castor husk waste as substrate is studied for alkaline protease production by Bacillus altitudinis GVC11 in solid-state fermentation. Various parameters like moisture content, incubation period, particle size, effect of carbon and nitrogen sources are studied and optimized for enzyme production. Highest enzyme production of 419,293 units per gram husk is obtained. Cost of enzyme production can be reduced by using castor husk as substrate.

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Acknowledgment

The authors thank the Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi, for financial assistance to carry out this work.

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Authors and Affiliations

  1. Department of Microbiology, Osmania University, Hyderabad, 500007, India

    A. Madhuri, B. Nagaraju, N. Harikrishna & Gopal Reddy

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  1. A. Madhuri
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  2. B. Nagaraju
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  3. N. Harikrishna
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  4. Gopal Reddy
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Correspondence to Gopal Reddy.

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Madhuri, A., Nagaraju, B., Harikrishna, N. et al. Production of Alkaline Protease by Bacillus altitudinis GVC11 using Castor Husk in Solid-State Fermentation. Appl Biochem Biotechnol 167, 1199–1207 (2012). https://doi.org/10.1007/s12010-012-9570-6

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  • DOI: https://doi.org/10.1007/s12010-012-9570-6

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