Acetone–butanol–ethanol (ABE) production from renewable resources has been widely reported. In this study, Clostridium butyricum EB6 was employed for ABE fermentation using fermentable sugar derived from treated oil palm empty fruit bunch (OPEFB). A higher amount of ABE (2.61 g/l) was produced in a fermentation using treated OPEFB as the substrate when compared to a glucose based medium that produced 0.24 g/l at pH 5.5. ABE production was increased to 3.47 g/l with a yield of 0.24 g/g at pH 6.0. The fermentation using limited nitrogen concentration of 3 g/l improved the ABE yield by 64%. The study showed that OPEFB has the potential to be applied for renewable ABE production by C. butyricum EB6.
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The authors are grateful for a National Science Fellowship (NSF) from the Ministry of Science, Technology and Innovation (MOSTI), Malaysia, and the Research University Grant Scheme (RUGS) from Universiti Putra Malaysia for financial support. We gratefully acknowledge the Fermentation Technology Unit, Institute of Bioscience UPM for equipment use and to all members of the Environmental Biotechnology Research Group for support.
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Ibrahim, M.F., Abd-Aziz, S., Razak, M.N.A. et al. Oil Palm Empty Fruit Bunch as Alternative Substrate for Acetone–Butanol–Ethanol Production by Clostridium butyricum EB6. Appl Biochem Biotechnol 166, 1615–1625 (2012). https://doi.org/10.1007/s12010-012-9538-6