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Enhanced Saccharification of Biologically Pretreated Wheat Straw for Ethanol Production

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Abstract

The biological pretreatment of lignocellulosic biomass with white-rot fungi for the production of bioethanol is an alternative to the most used physico-chemical processes. After biological treatment, a solid composed of cellulose, hemicellulose, and lignin—this latter is with a composition lower than that found in the initial substrate—is obtained. On the contrary, after applying physico-chemical methods, most of the hemicellulose fraction is solubilized, while cellulose and lignin fractions remain in the solid. The optimization of the combination of cellulases and hemicellulases required to saccharify wheat straw pretreated with the white-rot fungus Irpex lacteus was carried out in this work. The application of the optimal dosage made possible the increase of the sugar yield from 33 to 54 %, and at the same time the reduction of the quantity of enzymatic mixture in 40 %, with respect to the initial dosage. The application of a pre-hydrolysis step with xylanases was also studied.

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Acknowledgments

This work was economically supported by the CDTI (Project CEN-200910140) and by the Ministry of Economy and Competitiveness of Spain through the Local Investment Fund for Employment (Government of Spain). This research is carried out in collaboration with Abengoa Bionergía Nuevas Tecnologías. Authors thank also Novozymes for providing the commercial enzymes. T. Lu-Chau wishes to thank the I. Barreto Program from the Galicia Government for the economical support.

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Authors and Affiliations

  1. Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, Rúa Lope Gómez de Marzoa s/n, 15782, Santiago de Compostela, Spain

    M. López-Abelairas & T. A. Lu-Chau

  2. Department of Chemical Engineering, School of Engineering, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain

    J. M. Lema

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  1. M. López-Abelairas
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  2. T. A. Lu-Chau
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  3. J. M. Lema
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Correspondence to M. López-Abelairas.

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López-Abelairas, M., Lu-Chau, T.A. & Lema, J.M. Enhanced Saccharification of Biologically Pretreated Wheat Straw for Ethanol Production. Appl Biochem Biotechnol 169, 1147–1159 (2013). https://doi.org/10.1007/s12010-012-0054-5

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  • DOI: https://doi.org/10.1007/s12010-012-0054-5

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