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Production and Partial Characterization of Cellulases and Xylanases from Trichoderma atroviride 676 Using Lignocellulosic Residual Biomass

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Abstract

Trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. Best results were obtained when 3.0 % (w/v) untreated sugarcane bagasse was used (61.3 U mL−1 for xylanase, 1.9 U mL−1 for endoglucanase, 0.25 U mL−1 for FPase, and 0.17 U mL−1 for β-glucosidase) after 3–4 days fermentation. The maximal enzymatic activity for endoglucanase, FPase, and xylanase were observed at 50–60 °C and pH 4.0–5.0, whereas thermal stability at 50 °C (CMCase and FPase) or 40 °C (xylanase) was obtained after 8 h. Zymograms have shown two bands of 104 and 200 kDa for endoglucanases and three bands for xylanase (23, 36, and 55.7 kDa). The results obtained with T. atroviride strain 676 were comparable to those obtained with the cellulolytic strain Trichoderma reesei RUT-C30, indicating, in the studied conditions, its great potential for biotechnological application, especially lignocellulose biomass hydrolysis.

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Authors and Affiliations

  1. Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590, Brazil

    André Luiz Grigorevski-Lima, Mariana Menezes Quadros de Oliveira & Rosalie Reed Rodrigues Coelho

  2. Departamento de Engenharia Bioquímica, Escola de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil

    Rodrigo Pires do Nascimento

  3. Departamento de Bioquímica, Instituto de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil

    Elba Pinto da Silva Bon

Authors
  1. André Luiz Grigorevski-Lima
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  2. Mariana Menezes Quadros de Oliveira
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  3. Rodrigo Pires do Nascimento
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  4. Elba Pinto da Silva Bon
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  5. Rosalie Reed Rodrigues Coelho
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Correspondence to Rodrigo Pires do Nascimento.

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Grigorevski-Lima, A.L., de Oliveira, M.M.Q., do Nascimento, R.P. et al. Production and Partial Characterization of Cellulases and Xylanases from Trichoderma atroviride 676 Using Lignocellulosic Residual Biomass. Appl Biochem Biotechnol 169, 1373–1385 (2013). https://doi.org/10.1007/s12010-012-0053-6

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  • DOI: https://doi.org/10.1007/s12010-012-0053-6

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