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Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents

Applied Biochemistry and Biotechnology volume 169, pages 901–910 (2013)Cite this article

Abstract

In this study, the catalytic activity of aldehyde oxidase was investigated in various water-miscible organic solvents for the first time. The enzyme was partially purified from rabbit liver, and its activity was determined in the absence/presence of nine hydro-organic mixtures. The effects of pH and temperature on the enzyme activity were also investigated. The activity was reduced in the presence of all nine organic solvents. However, in some cases, the residual activity remained almost unchanged throughout the incubation of enzyme at 35 °C for 24 h. Considering potential advantages of doing reactions in the presence of organic solvents, these results could be of value. The enzyme showed different behavior in the reaction solutions making it difficult to formulate results in a single comprehensive model. The results indicated that binding and cleavage of the substrate are influenced in the presence of organic solvents.

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Affiliations

  1. Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran

    Mohammad-Reza Rashidi

  2. Biochemistry Department, Faculty of Natural Science, Payamnor University of Mashhad, Mashhad, Iran

    Moharam Dehghany

  3. Biochemistry Department, Faculty of Natural Science, Tabriz University, Tabriz, 51664-14766, Iran

    Golamreza Dehghan

  4. Pharmaceutical Chemistry Department, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran

    Abolghasem Jouyban & Akram Faridi

Authors
  1. Mohammad-Reza Rashidi
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  2. Moharam Dehghany
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  3. Golamreza Dehghan
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  4. Abolghasem Jouyban
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  5. Akram Faridi
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Correspondence to Mohammad-Reza Rashidi.

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Rashidi, MR., Dehghany, M., Dehghan, G. et al. Aldehyde Oxidase Activity and Stability in Water-Miscible Organic Solvents. Appl Biochem Biotechnol 169, 901–910 (2013). https://doi.org/10.1007/s12010-012-0036-7

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  • DOI: https://doi.org/10.1007/s12010-012-0036-7

Keywords

  • Aldehyde oxidase
  • Water-miscible organic solvents
  • Enzyme activity
  • Thermal stability
  • Kinetic constants