Abstract
Bacteria were isolated from wastewater containing highly concentrated hydrogen peroxide that had been used to clean the pure water delivery system in a semiconductor plant. One bacterium was selected for its high hydrogen peroxide degradation activity. In the presence of 1 % hydrogen peroxide, it degraded 72.5 % in 5 min. It showed 100 % viability after 6 h at 1 % hydrogen peroxide. Even at 3 % hydrogen peroxide, it survived for more than 6 h. This bacterium was named as Bacillus nitroreducens PLC9 since its 16S rRNA showed 100 % similarity with the recently reported new species B. nitroreducens. Purified catalase from B. nitroreducens PLC9 was characterized as a thermo-alkali-stable hydroperoxidase type II catalase, and it is suggested as a new type of catalase based on following: (1) it is stable over a broad pH range (pH 4–11); (2) it is consisted of homodimers with a molecular weight of 66 kDa (total molecular weight, 134 kDa); (3) its activity was not inhibited by 3-amino-1,2,4-triazole; and (4) its N-terminal sequence has never been reported before. Both B. nitroreducens PLC9 and the isolated catalase can be used for efficient degradation of hydrogen peroxide at high concentrations.
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This work was supported by a research grant from the National Research Foundation of Korea (Grant No. 511-2006-1-C00105).
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Hong, H., Bae, K.S. & Lee, Y. New Bacteria Bacillus nitroreducens PLC9 with Hydrogen Peroxide-Degrading Activity with High Survival Rate in Hydrogen Peroxide. Appl Biochem Biotechnol 169, 701–711 (2013). https://doi.org/10.1007/s12010-012-0034-9
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DOI: https://doi.org/10.1007/s12010-012-0034-9