Skip to main content
SpringerLink
Log in

Establishment and Quality Assessment of Tissue Cultures in Glycyrrhiza uralensis Fisch.

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

In this study, seedling, callus, cell, and adventitious root of Glycyrrhiza uralensis Fisch. have been established. In order to find the best one for producing G. uralensis active constituents, triterpenoid saponins and flavonoids in native root and tissue cultures were determined, and the contents in different G. uralensis materials were analyzed using cluster analysis. The contents of triterpenoid saponins and glycyrrhizic acid in tissue cultures were much lower than that in native G. uralensis. The total flavonoids content we determined in adventitious root was 6.31 mg g−1, which was close to that of native root (9.82 mg g−1). Based on the cluster analysis, we found that G. uralensis cultures were not suitable for production of glycyrrhizic acid, while adventitious root had a greater capability of flavonoids production comparing to seedling, callus, and cell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

KT:

Kinetin

NAA:

Naphthalene acetic acid

6-BA:

6-Benzylaminopurine

MS:

Murashige and Skoog

References

  1. Woo, T. C., Seo, H. L., & Jong, D. K. (2001). Cytotechnology, 37, 55–64.

    Article  Google Scholar 

  2. Valeria, M. D. M., & Maria, J. V. F. (2005). Journal of Pharmaceutical and Biomedical Analysis, 37, 287–295.

    Article  Google Scholar 

  3. Ohad, N., Jacob, V., Ramadan, M., & Sarit, I. (2003). Journal of Agricultural and Food Chemistry, 51, 1201–1207.

    Article  Google Scholar 

  4. Zhang, X. H., Zhao, Y. F., & Chen, J. M. (2001). China Journal of Chinese Materia Medica, 26–11, 746–747.

    Google Scholar 

  5. Murashige, T., & Skoog, F. (1962). Physiologia Plantarum, 15, 473–497.

    Article  CAS  Google Scholar 

  6. Lu, H. Y., Liu, Y., Zhang, H. C., & Gao, S. L. (2010). Chinese Journal of Modern Applied Pharmacy, 42–1, 43–46.

    Google Scholar 

  7. Bourgaud, F., Gravot, A., Milesi, S., & Gontier, E. (2001). Plant Science, 161, 839–851.

    Article  CAS  Google Scholar 

  8. Huang, T., Gao, W. Y., Wang, J., Zhao, Y. X., Huang, L. Q., & Liu, C. X. (2010). Minerva Biotecnologica, 22–2, 39–45.

    Google Scholar 

Download references

Acknowledgments

This research was funded by Science and Technology Commission of Tianjin, China (09ZCKFSH01100).

Author information

Authors and Affiliations

  1. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China

    Juan Wang & Wenyuan Gao

  2. Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, Tianjin, 300457, China

    Liming Zhang

  3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Luqi Huang

Authors
  1. Juan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenyuan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liming Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Luqi Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wenyuan Gao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, J., Gao, W., Zhang, L. et al. Establishment and Quality Assessment of Tissue Cultures in Glycyrrhiza uralensis Fisch.. Appl Biochem Biotechnol 169, 588–594 (2013). https://doi.org/10.1007/s12010-012-0012-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-0012-2

Keywords

Search

Navigation