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Xylitol Production by Genetically Engineered Trichoderma reesei Strains Using Barley Straw as Feedstock

Applied Biochemistry and Biotechnology volume 169, pages 554–569 (2013)Cite this article

Abstract

Xylitol, a naturally occurring five-carbon sugar alcohol derived from d-xylose, is currently in high demand by industries. Trichoderma reesei, a prolific industrial cellulase and hemicellulase producing fungus, is able to selectively use d-xylose from hemicelluloses for xylitol production. The xylitol production by T. reesei can be enhanced by genetic engineering of blocking further xylitol metabolism in the d-xylose pathway. We have used two different T. reesei strains which are impaired in the further metabolism of xylitol including a single mutant in which the xylitol dehydrogenase gene was deleted (∆xdh1) and a double mutant where additionally l-arabinitol-4-dehydrogenase, an enzyme which can partially compensate for xylitol dehydrogenase function, was deleted (∆lad1∆xdh1). Barely straw was first pretreated using NaOH and Organosolv pretreatment methods. The highest xylitol production of 6.1 and 13.22 g/L was obtained using medium supplemented with 2 % Organosolv-pretreated barley straw and 2 % d-xylose by the ∆xdh1 and ∆lad1∆xdh1 strains, respectively.

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Abbreviations

∆xdh1 :

Xylitol dehydrogenase gene deleted strain (T. reesei single mutant strain)

∆lad1∆xdh1 :

l-Arabinitol-4-dehydrogenase and xylitol dehydrogenase genes deleted strain (T. reesei double mutant strain)

PPP:

Pentose phosphate pathway

FTIR:

Fourier transform infrared spectroscopic

HPAE-PAD:

High-performance anion exchange chromatography with pulsed amperometric detection

DNS:

3,5-Dinitrosalicylic acid

FPU:

Filter paper unit

MCC:

Microcrystalline cellulose

RS:

Reducing sugars

References

  1. Dashtban, M., Schraft, H., & Qin, W. (2009). International Journal of Biological Sciences, 5, 578–595.

    Article  CAS  Google Scholar 

  2. Yu, C., Cao, Y., Zou, H., & Xian, M. (2011). Applied Microbiology and Biotechnology, 89, 573–583.

    Article  CAS  Google Scholar 

  3. Nygard, Y., Toivari, M. H., Penttila, M., Ruohonen, L., & Wiebe, M. G. (2011). Metabolic Engineering, 13, 383–391.

    Article  CAS  Google Scholar 

  4. Seiboth, B., & Metz, B. (2011). Applied Microbiology and Biotechnology, 89, 1665–1673.

    Article  CAS  Google Scholar 

  5. Taherzadeh, M. J., & Karimi, K. (2008). International Journal of Molecular Sciences, 9, 1621–1651.

    Article  CAS  Google Scholar 

  6. Dashtban, M., Schraft, H., Syed, T. A., & Qin, W. (2010). International Journal of Biochemistry and Molecular Biology, 1, 36–50.

    CAS  Google Scholar 

  7. Seiboth, B., Hartl, L., Pail, M., & Kubicek, C. P. (2003). Eukaryotic Cell, 2, 867–875.

    Article  CAS  Google Scholar 

  8. Seiboth, B., Gamauf, C., Pail, M., Hartl, L., & Kubicek, C. P. (2007). Molecular Microbiology, 66, 890–900.

    Article  CAS  Google Scholar 

  9. Sato, H., Ide, Y., Nasu, M., & Numabe, Y. (2011). Odontology, 99, 28–33.

    Article  CAS  Google Scholar 

  10. Prakasham, R., Rao, R., & Hobbs, P. (2009). Current Trends in Biotechnology and Pharmacy, 3, 8–36.

    CAS  Google Scholar 

  11. Santos, D. T., Sarrouh, B. F., Rivaldi, J. D., Converti, A., & Silva, S. S. (2008). Journal of Food Engineering, 86, 542–548.

    Article  CAS  Google Scholar 

  12. Khankal, R., Chin, J. W., & Cirino, P. C. (2008). Journal of Biotechnology, 134, 246–252.

    Article  CAS  Google Scholar 

  13. Ahmad, I., Shim, W. Y., Jeon, W. Y., Yoon, B. H., & Kim, J. H. (2012). Bioprocess and Biosystems Engineering, 35, 199–204.

    Article  CAS  Google Scholar 

  14. Akinterinwa, O., Khankal, R., & Cirino, P. C. (2008). Current Opinion in Biotechnology, 19, 461–467.

    Article  CAS  Google Scholar 

  15. El-Batal, I. A., & Khalaf, S. A. (2004). International Journal of Agriculture and Biology, 6, 1066–1073.

    CAS  Google Scholar 

  16. Rodrigues, R. C., Kenealy, W. R., & Jeffries, T. W. (2011). Journal of Industrial Microbiology and Biotechnology, 38, 1649–1655.

    Article  CAS  Google Scholar 

  17. Cheng, S., D’Cruz, I., Wang, M., Leitch, M., & Xu, M. (2010). Energy Fuels, 24, 4659–4667.

    Article  CAS  Google Scholar 

  18. Deshpande, P., Nair, S., & Khedkar, S. (2009). Applied Biochemistry and Biotechnology, 158, 552–560.

    Article  CAS  Google Scholar 

  19. Mandels, M. M., & Andreotti, R. E. (1978). Process Biochemistry, 13, 6–13.

    CAS  Google Scholar 

  20. Gamerith, G., Groicher, R., Zeilinger, S., Herzog, P., & Kubicek, C. P. (1992). Applied Microbiology and Biotechnology, 38, 315–322.

    Article  CAS  Google Scholar 

  21. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  22. Dashtban, M., Buchkowski, R., & Qin, W. (2011). International Journal of Biochemistry and Molecular Biology, 2, 274–286.

    CAS  Google Scholar 

  23. Dashtban, M., Maki, M., Leung, K. T., Mao, C., & Qin, W. (2010). Critical Reviews in Biotechnology, 30, 302–309.

    Article  CAS  Google Scholar 

  24. Dashtban, M., & Qin, W. (2012). Microbial Cell Factories, 11, 63.

    Article  CAS  Google Scholar 

  25. Vidal, B. C., Jr., Dien, B. S., Ting, K. C., & Singh, V. (2011). Applied Biochemistry and Biotechnology, 164, 1405–1421.

    Article  CAS  Google Scholar 

  26. Pedersen, M., & Meyer, A. S. (2009). Biotechnology Progress, 25, 399–408.

    Article  CAS  Google Scholar 

  27. da Silva, A. S., Inoue, H., Endo, T., Yano, S., & Bon, E. P. (2010). Bioresource Technology, 101, 7402–7409.

    Article  Google Scholar 

  28. Hsu, T. C., Guo, G. L., Chen, W. H., & Hwang, W. S. (2010). Bioresource Technology, 101, 4907–4913.

    Article  CAS  Google Scholar 

  29. Chundawat, S. P., Venkatesh, B., & Dale, B. E. (2007). Biotechnology and Bioengineering, 96, 219–231.

    Article  CAS  Google Scholar 

  30. Kumar, R., Mago, G., Balan, V., & Wyman, C. E. (2009). Bioresource Technology, 100, 3948–3962.

    Article  CAS  Google Scholar 

  31. Wang, B., Wang, X., & Feng, H. (2010). Bioresource Technology, 101, 752–760.

    Article  CAS  Google Scholar 

  32. Guo, G. L., Chen, W. H., Men, L. C., & Hwang, W. S. (2008). Bioresource Technology, 99, 6046–6053.

    Article  CAS  Google Scholar 

  33. Prathumpai, W., McIntyre, M., & Nielsen, J. (2004). Applied Microbiology and Biotechnology, 63, 748–753.

    Article  CAS  Google Scholar 

  34. Prakash, G., Varma, A. J., Prabhune, A., Shouche, Y., & Rao, M. (2011). Bioresource Technology, 102, 3304–3308.

    Article  CAS  Google Scholar 

  35. Pan, X., Gilkes, N., Kadla, J., Pye, K., Saka, S., Gregg, D., et al. (2006). Biotechnology and Bioengineering, 94, 851–861.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank Dr. Shuna Cheng and Dr. Chunbao (Charles) Xu for their great help with the Organosolv pretreatment. The authors would also like to thank Dr. Matthew Leitch for allowing us to use Wiley mill machine. This work was supported by a scholarship from Ontario Graduate Scholarship to M.D. and NSERC-RCD and Ontario Research Chair funding to W.Q.

Conflict of Interests

The authors declare that they have no competing interests.

Author information

Affiliations

  1. Biorefining Research Institute (BRI), Lakehead University, 955 Oliver Road, Thunder Bay, ON, Canada, P7E 5E1

    Mehdi Dashtban, Greg Kepka & Wensheng Qin

  2. Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, ON, Canada, P7E 5E1

    Mehdi Dashtban & Wensheng Qin

  3. Instrumentation Lab, Lakehead University, 955 Oliver Road, Thunder Bay, ON, Canada, P7E 5E1

    Greg Kepka

  4. Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria

    Bernhard Seiboth

Authors
  1. Mehdi Dashtban
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  2. Greg Kepka
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  3. Bernhard Seiboth
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  4. Wensheng Qin
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Corresponding author

Correspondence to Wensheng Qin.

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Dashtban, M., Kepka, G., Seiboth, B. et al. Xylitol Production by Genetically Engineered Trichoderma reesei Strains Using Barley Straw as Feedstock. Appl Biochem Biotechnol 169, 554–569 (2013). https://doi.org/10.1007/s12010-012-0008-y

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  • DOI: https://doi.org/10.1007/s12010-012-0008-y

Keywords

  • Agricultural residues
  • Trichoderma reesei
  • Xylitol production