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Strategies to Overcome Oxygen Transfer Limitations During Hairy Root Cultivation of Azadiracta indica for Enhanced Azadirachtin Production

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Abstract

The vast untapped potential of hairy root cultures as a stable source of biologically active chemicals has focused the attention of scientific community toward its commercial exploitation. However, the major bottleneck remains its successful scale-up. Due to branching, the roots form an interlocked matrix that exhibits resistance to oxygen transfer. Thus, present work was undertaken to develop cultivation strategies like optimization of inlet gas composition (in terms of % (v/v) O2 in air), air-flow rate and addition of oxygen vectors in the medium, to curb the oxygen transfer limitations during hairy root cultivation of Azadirachta indica for in vitro azadirachtin (a biopesticide) production. It was found that increasing the oxygen fraction in the inlet air (in the range, 20–100% (v/v) O2 in air) increased the azadirachtin productivity by approximately threefold, to a maximum of 4.42 mg/L per day (at 100% (v/v) O2 in air) with respect to 1.68 mg/L per day in control (air with no oxygen supplementation). Similarly, increasing the air-flow rate (in the range, 0.3–2 vvm) also increased the azadirachtin productivity to a maximum of 1.84 mg/L per day at 0.8 vvm of air-flow rate. On the contrary, addition of oxygen vectors (in the range, 1–4% (v/v); hydrogen peroxide, toluene, Tween 80, kerosene, silicone oil, and n-hexadecane), decreased the azadirachtin productivity with respect to control (1.76 mg/L per day).

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Madras, Chennai, 600 036, India

    Smita Srivastava

  2. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, 110 016, India

    Ashok Kumar Srivastava

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  1. Smita Srivastava
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  2. Ashok Kumar Srivastava
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Correspondence to Ashok Kumar Srivastava.

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Srivastava, S., Srivastava, A.K. Strategies to Overcome Oxygen Transfer Limitations During Hairy Root Cultivation of Azadiracta indica for Enhanced Azadirachtin Production. Appl Biochem Biotechnol 167, 1818–1830 (2012). https://doi.org/10.1007/s12010-011-9531-5

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  • DOI: https://doi.org/10.1007/s12010-011-9531-5

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