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Regulation of Xylanase Biosynthesis in Bacillus cereus BSA1

Applied Biochemistry and Biotechnology volume 167, pages 1052–1060 (2012)Cite this article

Abstract

Microbial xylanases have a promising biotechnological potential to be used in industries. In this study, regulation of xylanase production was examined in Bacillus cereus BSA1. Xylanase production was induced by xylan. The enzyme production further increased in the presence of xylose and arabinose in very low concentration with addition of xylan (0.5% up to 6.02 U/ml). Addition of glucose (about 0.1%) to the media supplemented with xylan repressed xylanase production. Even higher concentration (>0.1%) of xylose and arabinose repressed xylanase biosynthesis. Glucose-mediated repression was partially relived by addition of cyclic adenosine monophosphate. Chemical like 2-4-dinitrophenol, which can inhibit adenosine triphosphate synthesis in cell, repressed xylanase synthesis and it suggested xylanase synthesis to be an energy dependent process.

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Acknowledgments

Financial support by CSIR, Government of India is thankfully acknowledged.

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Affiliations

  1. Post Graduate Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, India

    Asish Mandal

  2. Department of Botany, Midnapore College, Midnapore, 721101, West Bengal, India

    Sanjay Kar

  3. Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India

    Pradeep Kumar Das Mohapatra, Chiranjit Maity, Bikas Ranjan Pati & Keshab Chandra Mondal

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  1. Asish Mandal
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  2. Sanjay Kar
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  3. Pradeep Kumar Das Mohapatra
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  4. Chiranjit Maity
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  5. Bikas Ranjan Pati
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  6. Keshab Chandra Mondal
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Correspondence to Pradeep Kumar Das Mohapatra.

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Mandal, A., Kar, S., Das Mohapatra, P.K. et al. Regulation of Xylanase Biosynthesis in Bacillus cereus BSA1. Appl Biochem Biotechnol 167, 1052–1060 (2012). https://doi.org/10.1007/s12010-011-9523-5

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  • DOI: https://doi.org/10.1007/s12010-011-9523-5

Keywords

  • Xylan
  • Xylanase
  • Regulation of biosynthesis
  • Bacillus cereus