Abstract
This work aimed at setting up a fully instrumented, laboratory-scale bioreactor enabling anaerobic valorization of solid substrates through hydrogen and/or volatile fatty acid (VFA) production using mixed microbial populations (consortia). The substrate used was made of meat-based wastes, especially from slaughterhouses, which are becoming available in large amounts as a consequence of the growing constraints for waste disposal from meat industry. A reconstituted microbial mesophilic consortium without Archaebacteria (methanogens), named PBr, was cultivated in a 5-L anaerobic bioreactor on slaughterhouse wastes. The experiments were carried out with sequential fed-batch operations, including liquid medium removal from the bioreactor and addition of fresh substrate. VFAs and nitrogen were the main metabolites observed, while hydrogen accumulation was very low and no methane production was evidenced. After 1,300 h of culture, yields obtained for VFAs reached 0.38 g/g dry matter. Strain composition of the microbial consortium was also characterized using molecular tools (temporal temperature gradient gel electrophoresis and gene sequencing).
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Acknowledgment
This work is a part of a scientific program supported by the French agency “Agence Nationale de la Recherche” (ANR) with the reference ANR-08-BIOE-013. The authors wish to thank partners of this programs, especially the company “Biobasic Environnement” and ADIV (Association pour le Développement de l’Industrie de la Viande) for giving slaughter wastes.
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Pessiot, J., Nouaille, R., Jobard, M. et al. Fed-batch Anaerobic Valorization of Slaughterhouse By-products with Mesophilic Microbial Consortia Without Methane Production. Appl Biochem Biotechnol 167, 1728–1743 (2012). https://doi.org/10.1007/s12010-011-9516-4
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DOI: https://doi.org/10.1007/s12010-011-9516-4