Applied Biochemistry and Biotechnology

, Volume 166, Issue 4, pp 1008–1019 | Cite as

Expression of Rice Thaumatin-Like Protein Gene in Transgenic Banana Plants Enhances Resistance to Fusarium Wilt

  • F. Mahdavi
  • M. Sariah
  • M. MaziahEmail author


The possibility of controlling Fusarium wilt—caused by Fusarium oxysporum sp. cubensec (race 4)—was investigated by genetic engineering of banana plants for constitutive expression of rice thaumatin-like protein (tlp) gene. Transgene was introduced to cauliflower-like bodies’ cluster, induced from meristemic parts of male inflorescences, using particle bombardment with plasmid carrying a rice tlp gene driving by the CaMV 35S promoter. Hygromycin B was used as the selection reagent. The presence and integration of rice tlp gene in genomic DNA confirmed by PCR and Southern blot analyses. RT-PCR revealed the expression of transgene in leaf and root tissues in transformants. Bioassay of transgenic banana plants challenged with Fusarium wilt pathogen showed that expression of TLP enhanced resistance to F. oxysporum sp. cubensec (race 4) compared to control plants.


Banana Fusarium wilt Male inflorescence Particle bombardment PR-5 Thaumatin-like protein 


  1. 1.
    Ploetz, R. C., & Pegg, K. G. (1997). Plant Pathology, 26, 239–249.Google Scholar
  2. 2.
    Ploetz, R. C., Thomas, J. E., & Slabaugh, W. R. (2003). In R. C. Poletz (Ed.), Diseases of tropical fruit crops, disease of banana and plantain (p. 544). Wallingford: CABI Publishing.CrossRefGoogle Scholar
  3. 3.
    Kim, Y. J., & Hwang, B. K. (2000). Physiologia Plantarum, 108, 51–60.Google Scholar
  4. 4.
    Sels, J., Mathys, J., De Coninck, B. M. A., Cammue, B. P. A., & De Bolle, M. F. C. (2008). Plant Physiology and Biochemistry, 46, 941–950.CrossRefGoogle Scholar
  5. 5.
    Edreva, A. (2005). General and Applied Plant Physiology, 31, 105–124.Google Scholar
  6. 6.
    Sarowar, S., Kim, Y. J., Kim, E. N., Kim, K. D., Hwang, B. K., Islam, R., et al. (2005). Plant Cell Reports, 24, 216–224.CrossRefGoogle Scholar
  7. 7.
    Yamamoto, T., Iketani, H., Ieki, H., Nishizawa, Y., Notsuka, K., Hibi, T., et al. (2000). Plant Cell Reports, 19, 639–646.CrossRefGoogle Scholar
  8. 8.
    Datta, K., Tu, J., Oliva, N., Ona, I., Velazhahan, R., Mew, T. W., et al. (2001). Plant Science, 160, 405–414.CrossRefGoogle Scholar
  9. 9.
    Sreeramanan, S., Maziah, M., Abdullah, M. P., Rosli, N. M., & Xavier, R. (2006). Biotechnology, 5, 189–197.CrossRefGoogle Scholar
  10. 10.
    Maziah, M., Sariah, M., & Sreeramanan, S. (2007). Plant Pathology Journal, 6, 271–282.CrossRefGoogle Scholar
  11. 11.
    Shin, S., Mackintosh, C. A., Lewis, J., Heinen, S. J., Radmer, L., Dill-Macky, R., et al. (2008). Journal of Experimental Botany, 59, 2371–2378.CrossRefGoogle Scholar
  12. 12.
    Datta, K., Velazhahan, R., Oliva, N., Ona, I., Mew, T., Khush, G. S., et al. (1999). Theoretical and Applied Genetics, 98, 1138–1145.CrossRefGoogle Scholar
  13. 13.
    Tobias, D. J., Manoharan, M., Pritsch, C., & Dahleen, L. S. (2007). Plant Cell Reports, 26, 631–639.CrossRefGoogle Scholar
  14. 14.
    Zhu, B., Chen, T. H. H., & Li, P. H. (1996). Planta, 198, 70–77.CrossRefGoogle Scholar
  15. 15.
    Chen, W. P., Chen, P. D., Liu, D. J., Kynast, R., Friebe, B., Velazhahan, R., et al. (1999). Theoretical and Applied Genetics, 99, 755–760.CrossRefGoogle Scholar
  16. 16.
    Li-Ping, X., Hua-Zhong, W., Zheng-Ning, J., Jin-Long, N., Ai-Zhong, C., Ling, Y., et al. (2008). Acta Agronomica Sinica, 34, 349–354.CrossRefGoogle Scholar
  17. 17.
    Jin, H., Yue, D. X., Zhou, L. Y., & Muthukrishnan, S. (2001). Acta Agriculturae Boreali Sinica, 16, 67–72.Google Scholar
  18. 18.
    Mahdavi, D. F., Sariah, M., Puad, M. P., & Maziah, M. (2010). African Journal of Biotechnology, 9, 2360–2366.Google Scholar
  19. 19.
    Dellaporta, S. L., Wood, J., & Hicks, J. B. A. (1983). Plant Molecular Biology, 1, 19–21.CrossRefGoogle Scholar
  20. 20.
    Sambrook, J., & Russell, D. W. (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.Google Scholar
  21. 21.
    Chen, W. P., Gu, X., Liang, G. H., Muthukrishnan, S., Chen, P. D., Liu, D. J., et al. (1998). Theoretical and Applied Genetics, 97, 1296–1306.CrossRefGoogle Scholar
  22. 22.
    Anand, A., Zhou, T., Trick, H. N., Gill, B. S., Bockus, W. W., & Muthukrishnan, S. (2003). Journal of Experimental Botany, 54, 1101–1111.CrossRefGoogle Scholar
  23. 23.
    Anand, A., Trick, H. N., Gill, B. S., & Muthukrishnan, S. (2003b) Stable transgene expression and random silencing in wheat. Plant Biotechnology Journal, 1, 241–251Google Scholar
  24. 24.
    Sreeramanan, S., Maziah, M., Rosli, N. M., Sariah, M., & Xavier, R. (2006). Biotechnology, 5, 203–216.CrossRefGoogle Scholar
  25. 25.
    Sagi, L., Panis, B., Remy, S., Schoofs, H., De Smet, K., Swennen, R., & Cammue, B. (1995) Genetic transformation of banana (Musa spp.) via particle bombardment. Bio/Technology, 13, 481–485.Google Scholar
  26. 26.
    Becker, D. K., Dugdale, B., Smith, M. K., Harding, R. M., & Dale, J. L. (2000). Plant Cell Reports, 19, 229–234.CrossRefGoogle Scholar
  27. 27.
    Matsumoto, K., Morais, L. S., Vianna, G. R., Aragão, J. R., & Rech, E. L. (2002). Acta Horticulturae, 575, 61–67.Google Scholar
  28. 28.
    Huang, X., Huang, X. L., Xiao, W., Zhao, J. T., Dai, X. M., Chen, Y. F., et al. (2007). Plant Cell Reports, 26, 1755–1762.CrossRefGoogle Scholar
  29. 29.
    Tripathi, L. (2005). In Thangadurai, T. Pullaiah, & L. Tripathi (Eds.), Genetic resources and biotechnology, transgenic technologies for developing bacterial disease resistance in plants (pp. 200–220). New Delhi: Regency.Google Scholar
  30. 30.
    Tripathi, L., Tripathi, J. N., & Hughes, J. D. A. (2005). African Journal of Biotechnology, 4, 1378–1383.Google Scholar
  31. 31.
    Iyer, L. M., Kumpatla, S. P., Chandrasekharan, M. B., & Hall, T. C. (2000). Plant Molecular Biology, 43, 323–346.CrossRefGoogle Scholar
  32. 32.
    Meyer, P., & Saedler, H. (1996). Annual Review of Plant Physiology and Plant Molecular Biology, 47, 23–48.CrossRefGoogle Scholar
  33. 33.
    Vaucheret, H., Beclin, C., Elmayan, T., Feuerbach, F., Godon, C., Morel, J. B., et al. (1998). The Plant Journal, 16, 651–659.CrossRefGoogle Scholar
  34. 34.
    Altpeter, F., Baisakh, N., Beachy, R., Bock, R., Capell, T., Christou, P., et al. (2005). Molecular Breeding, 15, 305–327.CrossRefGoogle Scholar
  35. 35.
    Fu, X., Kohli, A., Twyman, R. M., & Christou, P. (2000). Molecular & General Genetics, 263, 106–118.CrossRefGoogle Scholar
  36. 36.
    Liu, D., Raghothama, K. G., Hasegawa, P. M., & Bressan, R. A. (1994). Proceedings of the National Academy of Sciences of the United States of America, 91, 1888–1892.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Institute of Tropical AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Department of Biochemistry, Faculty of Biotechnology and Molecular SciencesUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Plant protection, Faculty of AgricultureUniversiti Putra MalaysiaSerdangMalaysia

Personalised recommendations