Skip to main content

Advertisement

Springer Nature Link
Log in

Determination of Dimethyl Phthalate in Environment Water Samples by a Highly Sensitive Indirect Competitive ELISA

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Recent controversy over the discovery of clouding agents containing the banned chemical di(2-ethylhexyl) phthalate in beverages in 2011 in Taiwan has caused public concerns. For the detection of dimethyl phthalate (DMP) in environment water samples, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed in this paper. Dimethyl 4-aminophthalate (4-DMAP) was covalently attached to bovine serum albumin as immunogen by a diazotization method. The conjugation of DMAP and ovalbumin as coating antigen was obtained in the same way. Polyclonal antibody was obtained from New Zealand white rabbits. Under the optimized conditions, DMP was detected in the concentration range of 0.02–419 ng/mL with a detection limit of 0.01 ng/mL. The proposed method has been applied to the analysis of river water, lake water, and rain water samples. Satisfactory recoveries were obtained ranging from 90.6% to 105.5%. The cross-reactivities of the anti-DMP antibody to seven structurally related phthalate esters were below 10%. The data demonstrated that the ic-ELISA method described in our study is a simple, sensitive, and specific method and showed that this assay is a reliable tool to detect DMP in water samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. Feng, Y. L., Zhu, J. P., & Sensenstein, R. (2005). Analytica Chimica Acta, 538, 41–48.

    Article  CAS  Google Scholar 

  2. Howdeshell, K. L., Rider, C. V., Wilson, V. S., & Gray, L. E., Jr. (2008). Environmental Research, 108(2), 168–176.

    Article  CAS  Google Scholar 

  3. Simoneit, B. R. T., Medeiros, T. P., & Didyk, B. (2005). Environmental Science & Technology, 39, 6961–6970.

    Article  CAS  Google Scholar 

  4. Heudorf, U., Mersch-Sundermann, V., & Angerer, J. (2007). International Journal of Hygiene and Environmental Health, 210(5), 623–634.

    Article  CAS  Google Scholar 

  5. Zeng, F., Wen, J. X., Cui, K. Y., Wu, L. N., Liu, M., Li, Y. J., et al. (2009). Journal of Hazardous Materials, 169(2), 719–725.

    Article  CAS  Google Scholar 

  6. Liang, P., Xu, J., & Li, Q. (2008). Analytica Chimica Acta, 609(1), 53–58.

    Article  CAS  Google Scholar 

  7. Zeng, F., Cui, K. Y., Xie, Z. Y., Wu, L. N., Luo, D. L., Chen, L. X., et al. (2009). Journal of Hazardous Materials, 164(3), 1171–1178.

    Article  CAS  Google Scholar 

  8. Ostrovsky, I., Cabala, R., Kubinec, R., Gorova, R., Blaško, J., Kubincova, J., et al. (2011). Food Chemistry, 124(1), 392–395.

    Article  CAS  Google Scholar 

  9. Su, R., Zhao, X. W., Li, Z. Y., Jia, Q., Liu, P., & Jia, J. B. (2010). Analytica Chimica Acta, 676(2), 103–108.

    Article  CAS  Google Scholar 

  10. Main, K. M., Mortensen, G. K., Kaleva, M. M., Boisen, K. A., Damgaard, I. N., Chellakooty, M., et al. (2006). Environmental Health Perspectives, 114(2), 270–276.

    Article  CAS  Google Scholar 

  11. Chen, W. L., & Sung, H. H. (2005). Aquatic Toxicology, 74(2), 160–171.

    Article  CAS  Google Scholar 

  12. Ema, M., Miyawaki, E., & Kawashima, K. (1998). Toxicology Letters, 98, 87–93.

    Article  CAS  Google Scholar 

  13. Barr, D. B., Silva, M. J., Kato, K., Reidy, J. A., Malek, N. A., Hurtz, D., et al. (2003). Environmental Health Perspectives, 111(9), 1148–1151.

    Article  Google Scholar 

  14. Duty, S. M., Singh, N. P., Silva, M. J., Barr, D. B., Brock, J. W., Ryan, L., et al. (2003). Environmental Health Perspectives, 111(9), 1164–1169.

    Article  CAS  Google Scholar 

  15. Cai, Q. Y., Mo, C. H., Wu, Q. T., Zeng, Q. Y., & Katsoyiannis, A. (2007). Chemosphere, 68, 1751–1762.

    Article  CAS  Google Scholar 

  16. Mo, C. H., Cai, Q. Y., Li, Y. H., & Zeng, Q. Y. (2008). Journal of Hazardous Materials, 152, 1208–1213.

    Article  CAS  Google Scholar 

  17. Farahani, H., Norouzi, P., Dinarvand, R., & Ganjali, M. R. (2007). Journal of Chromatography. A, 1172, 105–112.

    Article  CAS  Google Scholar 

  18. Lequin, R. M. (2005). Clinical Chemistry, 51, 2415–2418.

    Article  CAS  Google Scholar 

  19. Ius, A., Bacigalupo, M. A., Meroni, G., Pistillo, A., & Roda, A. (1993). Fresenius’ Journal of Analytical Chemistry, 345, 589–591.

    Article  CAS  Google Scholar 

  20. Zhang, M. C., Wang, Q. E., & Zhuang, H. S. (2006). Analytical and Bioanalytical Chemistry, 386, 1401–1406.

    Article  CAS  Google Scholar 

  21. Zhang, M. C., Wang, Q. E., & Zhuang, H. S. (2007). Analytical Letters, 40, 127–137.

    Article  Google Scholar 

  22. Wei, C. X., Zhao, W., Zhang, Y. B., Yu, X. W., & Yuan, J. L. (2009). 3rd International Conference on Bioinformatics and Biomedical Engineering, 1–4.

  23. Zhang, M. C., Liu, B. L., Cong, Y., Liu, S. H., & Hu, Y. R. (2011). Food and Agricultural Immunology. doi:10.1080/09540105.2011.575124.

  24. Zhang, M. C., Cong, Y., Sheng, Y. L., & Liu, B. L. (2010). Analytical Biochemistry, 406(1), 24–28.

    Article  CAS  Google Scholar 

  25. Zhang, M. C., & Sheng, Y. L. (2010). Journal of Fluorescence, 20, 1167–1173.

    Article  CAS  Google Scholar 

  26. Sun, M. M., Du, L. Y., Gao, S. Q., Bao, Y. H., & Wang, S. H. (2010). Steroids, 75, 400–403.

  27. Gabaldón, J. A., Maquieira, A., & Puchades, R. (1999). Critical Reviews in Food Science and Nutrition, 39(6), 519–538.

    Article  Google Scholar 

  28. Casino, P., Morais, S., Puchades, R., & Maquieira, A. (2001). Environmental Science & Technology, 35, 4111–4119.

    Article  CAS  Google Scholar 

  29. Maria-Pilar, M., Gee, S., & Hammock, B. D. (1995). Trends in Analytical Chemistry, 14, 415–425.

    Google Scholar 

  30. Liang, B., Zhang, Y. X., Liu, A. P., Zhou, Y. X., Chen, F. S., & Wang, X. H. (2011). Applied Biochemistry and Biotechnology, 164(6), 831–840.

    Article  CAS  Google Scholar 

  31. Rodriguez-Mozaz, S., Reder, S., Lopez de Alda, M., Gauglitz, G., & Barceló, D. (2004). Biosensors and Bioelectronics, 19(7), 633–640.

    Article  CAS  Google Scholar 

  32. Kawaguchi, T., Shankaran, D. R., Kim, S. J., Gobi, K. V., Matsumoto, K., Toko, K., et al. (2007). Talanta, 72(2), 554–560.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (numbers 21075002, 21175004 and 21177082), the Science and Technology Commission of Shanghai Municipality in China (the key project of fundamental research number 09JC1407600), and the Key Program of the Natural Science Foundation of the Anhui Higher Education Institutions of China (grant number KJ2011A141). We express our deep thanks for the financial support for this study.

Author information

Authors and Affiliations

  1. Key Laboratory of Chemo-Biosensing, Anhui Province, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, Anhui Province, 241000, China

    Mingcui Zhang, Shaohui Liu & Yurong Hu

  2. School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai, 200240, China

    Huisheng Zhuang

Authors
  1. Mingcui Zhang
    View author publications

    Search author on:PubMed Google Scholar

  2. Shaohui Liu
    View author publications

    Search author on:PubMed Google Scholar

  3. Huisheng Zhuang
    View author publications

    Search author on:PubMed Google Scholar

  4. Yurong Hu
    View author publications

    Search author on:PubMed Google Scholar

Corresponding authors

Correspondence to Mingcui Zhang or Huisheng Zhuang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, M., Liu, S., Zhuang, H. et al. Determination of Dimethyl Phthalate in Environment Water Samples by a Highly Sensitive Indirect Competitive ELISA. Appl Biochem Biotechnol 166, 436–445 (2012). https://doi.org/10.1007/s12010-011-9439-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9439-0

Keywords