Optimization of Laccase Production by Trametes versicolor Cultivated on Industrial Waste
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Laccases are very interesting biocatalysts for several industrial applications. Its production by different white-rot fungi can be stimulated by a variety of inducing substrates, and the use of lignocellulosic wastes or industrial by-products is one of the possible approaches to reduce production costs. In this work, various industrial wastes were tested for laccase production by Trametes versicolor MZKI G-99. Solid waste from chemomechanical treatment facility of a paper manufacturing plant showed the highest potential for laccase production. Enzyme production during submerged cultivation of T. versicolor on the chosen industrial waste has been further improved by medium optimization using genetic algorithm. Concentrations of five components in the medium were optimized within 60 shake-flasks experiments, where the highest laccase activity of 2,378 U dm−3 was achieved. Waste from the paper industry containing microparticles of CaCO3 was found to stimulate the formation of freely dispersed mycelium and laccase production during submerged cultivation of T. versicolor. It was proven to be a safe and inexpensive substrate for commercial production of laccase and might be more widely applicable for metabolite production by filamentous fungi.
KeywordsLaccase production Industrial waste Medium optimization Genetic algorithm Trametes versicolor Microparticles
This work was supported by the Croatian Ministry of Science, Education, and Sports (contract grant number 125-1252086-2793) and by The National Foundation for Science, Higher Education, and Technological Development of the Republic of Croatia (Program NZZ Installation Grant). P. Žnidaršič Plazl was supported by grant P2-0191, provided by the Ministry of Higher Education, Science, and Technology of the Republic of Slovenia. The authors gratefully acknowledges Dr. D. Ravnjak for the provision of CaCO3, sludge, and pulps from Papirnica Vevče, Ljubljana, Slovenia; Mrs. I. Škraba for providing microorganism from the Microbial Culture Collection of the National Institute of Chemistry, Slovenia; Mrs. Mira Špehar for the provision of the waste from the malt industry and barley husk, Slavonija slad d.o.o., Nova Gradiška, Croatia; and Mr. Dean Pinjuh for the provision of sawdusts, Hrvatske šume d.o.o., Slavonski Brod, Croatia. The authors wish to thank Mrs. Nataša Car for the skilled technical assistance.
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