Abstract
1,3-Propanediol (1,3-PD) biosynthesis plays a key role in NADH consumption to regulate the intracellular reducing equivalent balance of Klebsiella pneumoniae. This study aimed to increase reducing equivalent for enhancing 1,3-PD production through cofermentation of glycerol and xylose. Adding xylose as cosubstrate resulted in more reducing equivalent generation and higher cell growth. In batch fermentation under microaerobic condition, the 1,3-PD concentration, conversion from glycerol, and biomass (OD600) relative to cofermentation were increased significantly by 9.1%, 20%, and 15.8%, respectively. The reducing equivalent (NADH) was increased by 1–3 mg/g (cell dry weight) compared with that from glycerol alone. Furthermore, 2,3-butannediol was also doubly produced as major byproduct. In fed-batch fermentation with xylose as cosubstrate, the final 1,3-PD concentration, conversion from glycerol, and productivity were improved evidently from 60.78 to 67.21 g/l, 0.52 to 0.63 mol/mol, and 1.64 to1.82 g/l/h, respectively.
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This work was financially supported by the key program of National Natural Science Foundation of China (No. 200936002), the National Basic Research Program of China (No. 2011CB710800), and the National High Technology Research and Development Program of China (No. 2006AA020103).
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Jin, P., Lu, Sg., Huang, H. et al. Enhanced Reducing Equivalent Generation for 1,3-Propanediol Production Through Cofermentation of Glycerol and Xylose by Klebsiella pneumoniae . Appl Biochem Biotechnol 165, 1532–1542 (2011). https://doi.org/10.1007/s12010-011-9373-1
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DOI: https://doi.org/10.1007/s12010-011-9373-1