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Direct and Indirect Organogenesis of Alpinia galanga and the Phytochemical Analysis

Applied Biochemistry and Biotechnology volume 165, pages 1366–1378 (2011)Cite this article

Abstract

Alpinia galanga is a rhizomatous herb rich in essential oils and various other significant phytoconstituents. Rapid direct regeneration was obtained from the rhizome explants (15.66 ± 0.57 shoots) on MS media supplemented with zeatin at a concentration of 2 mg/l. The callus cultures of A. galanga were initiated from the rhizome explants on MS media supplemented with 2 mg/l each of BAP, 2,4-D, and NAA. The callus was analyzed for the presence of a vital phytoconstituent—acetoxychavicol acetate (ACA) associated with various biological properties. ACA was detected in the young friable callus as well as the stationary phase callus. Moreover, the induction of morphogenetic response in callus resulted in higher accumulation of ACA. The phytohormone withdrawal from the propagation media and the subsequent transfer of callus to BAP (2 mg/l) containing MS media has resulted in multiple shoot induction. The regenerated (indirect) plants have shown 1.6-fold higher ACA content (1.253%) when compared to the control plant (0.783%). Micropropagation of such conventionally propagated plants is very essential to meet the commercial demand as well as to ensure easy storage and transportation of disease free stocks.

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Abbreviations

MS:

Murashige and Skoog

BAP:

Benzyl amino purine

2,4-D:

2,4-Dichlorophenoxy acetic acid

TDZ:

Thidiazuron

NAA:

α-Naphthalene acetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

ACA:

Acetoxychavicol acetate

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Acknowledgement

The authors are grateful to the financial assistance provided by Department of Science & Technology under the Women Scientist Scheme (WOS-A) to Ms. Kiranmayee Rao.

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Affiliations

  1. Centre for Biotechnology, Institute of Science & Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad, 500 085, India

    Kiranmayee Rao, Bhuvaneswari Chodisetti, Suryakala Gandi, Lakshmi Narasu Mangamoori & Archana Giri

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  1. Kiranmayee Rao
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  2. Bhuvaneswari Chodisetti
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  3. Suryakala Gandi
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  4. Lakshmi Narasu Mangamoori
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Correspondence to Archana Giri.

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Rao, K., Chodisetti, B., Gandi, S. et al. Direct and Indirect Organogenesis of Alpinia galanga and the Phytochemical Analysis. Appl Biochem Biotechnol 165, 1366–1378 (2011). https://doi.org/10.1007/s12010-011-9353-5

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Keywords

  • Alpinia galanga
  • Rhizome
  • Callus
  • Micropropagation
  • Acetoxychavicol acetate
  • HPLC