Characterization and Identification of a Chymotryptic Hydrolysate of Alpha-Lactalbumin Stimulating Cholecystokinin Release in STC-1 Cells
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Alpha-lactalbumin hydrolysate is of significant interest, due to its potential application as a source of bioactive peptides in nutraceutical and pharmaceutical domains. This study was focused on the cholecystokinin (CCK) family compounds which are small peptides involved in the satiety control. The action of chymotryptic hydrolysate of alpha-lactalbumin on cholecystokinin release from intestinal endocrine STC-1 cells was investigated. We demonstrated for the first time that a chymotryptic hydrolysate of alpha-lactalbumin was able to highly stimulate CCK-releasing activity from STC-1 cells. The peptidic hydrolysate was characterized by LC/MS and MS/MS, thus highlighting the presence of 11 fractions containing 21 peptides, each potentially having the desired activity.
KeywordsAlpha-lactalbumin hydrolysate Cholecystokinin Enteroendocrine STC-1 cells Peptides
We gratefully acknowledge Dr Rozenn Ravallec from the Laboratoire ProBioGEM (Villeneuve d’Ascq, France) for her helpful involvement in the development of in vitro tests of CCK-releasing activity in our laboratory, tests used in the present work.
- 9.Chiba, H., & Yoshikawa, M. (1986). Protein Tailoring for Food Medical Uses (pp. 123–153). Marcel Dekker: New York.Google Scholar
- 11.Pellegrini, A., Thomas, U., Bramaz, N., Hunziker, P. V., & Fellenberg, R. (1999). Biochimica et Biophysica Acta, 1426, 439–448.Google Scholar
- 13.Baile, C. A., McLaughlin, C. L., & Della-Fera, M. A. (1986). Physiological Reviews, 66, 172–234.Google Scholar
- 18.Nakajima, K., Powers, J. C., Ashe, B. M., & Zimmerman, M. (1979). Journal of Biological Chemistry, 254, 4027–4032.Google Scholar
- 19.Lignot, B., Froidevaux, R., Nedjar-Arroume, N., & Guillochon, D. (1999). Biotechnology and Applied Biochemistry, 30, 201–207.Google Scholar
- 21.Rindi, G., Grant, S. G., Yiangou, Y., Ghatei, M. A., Bloom, S. R., Bautch, V. L., et al. (1990). American Journal of Pathology, 136, 1349–1363.Google Scholar
- 24.Keil, B. (1987). Protein Sequences & Data Analysis, 1, 13–20.Google Scholar