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Evaluation of Ethanol Production from Corncob Using Scheffersomyces (Pichia) stipitis CBS 6054 by Volumetric Scale-up

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Abstract

In scale-up, the potential of ethanol production by dilute sulfuric acid pretreatment using corncob was investigated. Pretreatments were performed at 170 °C with various acid concentrations ranging from 0% to 1.656% based on oven dry weight. Following pretreatment, pretreated biomass yield ranged from 59% to 67%. More than 90% of xylan was removed at 0.828% of sulfuric acid. At same pretreatment condition, the highest glucose yield obtained from pretreated biomass by enzymatic hydrolysis was about 76%, based on a glucan content of 37/100 g. In hydrolysate obtained by pretreatment, glucose concentration was low, while xylose concentration was significantly increased above 0.368% of sulfuric acid. At 1.656% of sulfuric acid, xylose and glucose concentration was highest. In subsequent, fermentation with hydrolysate, maximal ethanol yield was attained after 24 h with 0.368% of sulfuric acid. The fermentation efficiency of hydrolysate obtained by enzymatic hydrolysis reached a maximum of 75% at an acid charge of 0.368%.

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References

  1. Barl, B., Biliaderis, C. G., Murray, E. D., & Macgregor, A. W. (1991). Combined chemical and enzymatic treatment of corn husks lignocellulosics. Journal of the Science of Food and Agriculture, 56, 195–214.

    Article  CAS  Google Scholar 

  2. Becall, D. S., & Ingram, L. O. (1992). Conversion of hydrolysate of corncobs and hulls into ethanol by recombinant Escherichia coli B containing integrated genes for ethanol production. Biotechnology Letters, 14, 857–862.

    Article  Google Scholar 

  3. Cao, N. J., Krishnan, M. S., Du, J. X., Gong, C. S., Ho, N. W. Y., & Chen, Z. D. (1996). Ethanol production from corncob pretreated by the ammonia steeping process using genetically engineered yeast. Biotechnology Letters, 18, 1013–1018.

    Article  CAS  Google Scholar 

  4. Chen, Y., Dong, B., Qin, W., & Xiao, D. (2010). Xylose and cellulose fractionation from corncob with three different strategies and separate fermentation of them to bioethanol. Bioresource Technology, 101, 6994–6999.

    Article  CAS  Google Scholar 

  5. Himmel, M. E., Ding, S. Y., Johnson, D. K., Adney, W. S., Nimlos, M. R., & Brady, J. W. (2007). Biomass recalcitrance: Engineering plants and enzymes for biofuels production. Science, 315, 804–807.

    Article  CAS  Google Scholar 

  6. Larsson, S., Palmqvist, E., Hahn-Hagerdal, B., Tengborg, C., Stenberg, K., & Zacchi, G. (1999). The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme and Microbial Technology, 24, 151–159.

    Article  CAS  Google Scholar 

  7. Lee, J. W., Rodrigues, R. C. L. B., & Jeffries, T. W. (2009). Simultaneous saccharification and ethanol fermentation of corncob pretreated with oxalic acid using response surface methodology. Bioresource Technology, 100, 6307–6311.

    Article  CAS  Google Scholar 

  8. Liu, K., Lin, L., Yue, J., Li, X., Fang, X., & Zhu, M. (2010). High concentration ethanol production from corncob residues by fed-batch strategy. Bioresource Technology, 101, 4952–4958.

    Article  CAS  Google Scholar 

  9. Shen, Y., Zhang, Y., Ma, T., Bao, X., Du, F., Zhuang, G., et al. (2008). Simultaneous saccharification and fermentation of acid-pretreated corncobs with recombinant Saccharomyces cerevisiae expressing beta-glucosidase. Bioresource Technology, 99, 5099–5103.

    Article  CAS  Google Scholar 

  10. Wang, G. S., Lee, J.-W., Zhu, J. Y., & Jeffries, T. W. (2011). Dilute acid pretreatment of corncob for efficient sugar production. Applied Biochemisty and Biotechnology, 163, 658–668.

    Article  CAS  Google Scholar 

  11. Zhang, M., Wang, F., Su, R., Qi, W., & He, Z. (2010). Ethanol production from high dry matter corncob using fed-batch simultaneous saccharification and fermentation after combined pretreatment. Bioresource Technology, 101, 4959–4964.

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by Priority Research Centers Programs through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (Project No. 2011-0018393) to J.-W. Lee and by EdenIQ under CRADA 08-RD-11111126-007 with the Forest Products Laboratory. The authors thank Frederick J. Matt of the Analytical Chemistry and Microscopy Laboratory of the USDA Forest Products Laboratory (FPL) for carrying out the carbohydrate determination.

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Authors and Affiliations

  1. Department of Forest Products and Technology (BK21 Program), College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, South Korea

    Jae-Won Lee

  2. Bioenergy Research Center, Chonnam National University, Gwangju, 500-757, South Korea

    Jae-Won Lee

  3. US Forest Service, Forest Products Laboratory, Madison, WI, USA

    J. Y. Zhu & Thomas W. Jeffries

  4. Dipartimento di Scienze delle Produzioni Agrarie e Alimentari-DISPA, University of Catania, Via Valdisavoia 5, 95123, Catania, Italy

    Danilo Scordia

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  1. Jae-Won Lee
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  2. J. Y. Zhu
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  4. Thomas W. Jeffries
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Correspondence to Jae-Won Lee.

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Lee, JW., Zhu, J.Y., Scordia, D. et al. Evaluation of Ethanol Production from Corncob Using Scheffersomyces (Pichia) stipitis CBS 6054 by Volumetric Scale-up. Appl Biochem Biotechnol 165, 814–822 (2011). https://doi.org/10.1007/s12010-011-9299-7

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  • DOI: https://doi.org/10.1007/s12010-011-9299-7

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