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Comparison of Denitrification Between Paracoccus sp. and Diaphorobacter sp.

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Abstract

Denitrification was compared between Paracoccus sp. and Diaphorobacter sp. in this study, both of which were isolated from activated sludge of a denitrifying reactor. Denitrification of both isolates showed contrasting patterns, where Diaphorobacter sp. showed accumulation of nitrite in the medium while Paracoccus sp. showed no accumulation. The nitrate reduction rate was 1.5 times more than the nitrite reduction in Diaphorobacter sp., as analyzed by the resting state denitrification kinetics. Increasing the nitrate concentration in the medium increased the nitrite accumulation in Diaphorobacter sp., but not in Paracoccus sp., indicating a branched electron transfer during denitrification. Diaphorobacter sp. was unable to denitrify efficiently at high nitrate concentrations from 1 M, but Paracoccus sp. could denitrify even up to 2 M nitrate. Paracoccus sp. was found to be an efficient denitrifier with insignificant amounts of nitrite accumulation, and it could also denitrify high amounts of nitrate up to 2 M. Efficient denitrification without accumulation of intermediates like nitrite is desirable in the removal of high nitrates from wastewaters. Paracoccus sp. is shown to suffice this demand and could be a potential organism to remove high nitrates effectively.

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Acknowledgment

This study was supported by Gujarat State Biotechnology Mission (GSBTM) grant (GSBTM/MD/Projects/1450/2004-05).

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  1. Department of Microbiology and Biotechnology Centre, Faculty of Science, The M. S. University of Baroda, Vadodara, Gujarat, India

    Srinandan S. Chakravarthy, Samay Pande, Ashish Kapoor & Anuradha S. Nerurkar

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  1. Srinandan S. Chakravarthy
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  2. Samay Pande
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  3. Ashish Kapoor
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  4. Anuradha S. Nerurkar
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Correspondence to Anuradha S. Nerurkar.

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Chakravarthy, S.S., Pande, S., Kapoor, A. et al. Comparison of Denitrification Between Paracoccus sp. and Diaphorobacter sp.. Appl Biochem Biotechnol 165, 260–269 (2011). https://doi.org/10.1007/s12010-011-9248-5

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