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Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8

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Abstract

This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50 μg/mL) reduced the population of B. cereus from >108 to 104 colony-forming units per milliliter within 30 min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (no. 2009–0073489).

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Authors and Affiliations

  1. Department of Biotechnology (BK21 Program), Chung-Ang University, An-seong, 456-756, Republic of Korea

    Nam Keun Lee, In-Cheol Yeo, Joung Whan Park & Young Tae Hahm

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  1. Nam Keun Lee
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  2. In-Cheol Yeo
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  3. Joung Whan Park
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  4. Young Tae Hahm
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Correspondence to Young Tae Hahm.

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Lee, N.K., Yeo, IC., Park, J.W. et al. Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8. Appl Biochem Biotechnol 165, 235–242 (2011). https://doi.org/10.1007/s12010-011-9246-7

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