Skip to main content
SpringerLink
Log in

Studies on Immobilization and Partial Characterization of Lipases from Wheat Seeds (Triticum aestivum)

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The objective of this study was to provide some features on immobilization and partial characterization of lipases from wheat seeds. The optimum pH and temperature were found to be 5.5 and 32–37 °C, respectively. The stability of the concentrated enzymatic extract to high temperatures (25, 35, 45, and 55 °C) showed that the incubation of the extract at 55 °C led to its complete inactivation. The concentrated enzymatic extract kept 90% of its hydrolytic and esterification activities until 70 and 40 days of storage at 4 °C, respectively. The extract presented higher hydrolytic specificity to substrates of medium and long chains and higher esterification affinity to fatty acids of short and medium chains and alcohols with two and three carbon atoms. After the immobilization process using activated coal and sodium alginate as supports, an enhancement of about threefold in lipase activity was observed. The development of the present work permitted us to point out some characteristics of lipases from wheat seeds necessary for the proposition of new future industrial applications for this important biocatalyst.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Jaeger, K. E., & Reetz, M. T. (1998). Trends in Biotechnology, 16, 396–403.

    Article  CAS  Google Scholar 

  2. Mukherjee, K. D., & Kiewitt, I. (1996). Journal of Agricultural and Food Chemistry, 44, 1948–1952.

    Article  CAS  Google Scholar 

  3. Paques, F. W., & Macedo, G. A. (2006). Quimica Nova, 29, 93–99.

    Article  CAS  Google Scholar 

  4. Hasan, F., Shah, A. A., & Hameed, A. (2006). Enzyme and Microbial Technology, 39, 235–251.

    Article  CAS  Google Scholar 

  5. Villeneuve, P. (2003). European Journal of Lipid Science and Technology, 105, 308–317.

    Article  CAS  Google Scholar 

  6. Treichel, H., Oliveira, D., Mazutti, M. A., Di Luccio, M., & Oliveira, J. V. (2010). Food and Bioprocess Technology, 3, 182–188.

    Article  CAS  Google Scholar 

  7. Peter, F., & Preda, G. (2002). Journal of Molecular Catalysis B Enzymatic, 19–20, 467–472.

    Article  Google Scholar 

  8. Sun, S. Y., & Xu, Y. (2009). Bioresource Technology, 100, 1336–1342.

    Article  CAS  Google Scholar 

  9. Menoncin, S., Domíngues, N. M., Freire, D. M. G., Toniazzo, G., Cansian, R. L., Oliveira, J. V., et al. (2010). Food and Bioprocess Technology, 3, 461–465.

    Article  Google Scholar 

  10. Illanes, A. (1994). Biotecnologia de enzimas. Chile: Ediciones Universitarias de Valparaíso de la Universidad Católica de Valparaíso.

    Google Scholar 

  11. Cavalcanti, E. D. C., Gutarra, M. L. E., Freire, D. M. G., Castilho, L. R., & Júnior, G. L. S. (2005). Brazilian Archives of Biology and Technology, 48, 79–84.

    Article  Google Scholar 

  12. Pierozan, M. K., Costa, R. J., Antunes, O. A. C., Oestreicher, E. G., Oliveira, J. V., Cansian, R. L., et al. (2009). Journal of Agricultural and Food Chemistry, 57, 9716–9721.

    Article  CAS  Google Scholar 

  13. Freire, D. M. G., Gomes, M. P., & Bom, S. P. E. (1997). Journal of the Brazilian Society for Microbiology, 28, 6–12.

    Google Scholar 

  14. Oliveira, D., Feihrmann, A. C., Dariva, C., Cunha, A. G., Bevilaqua, J. V., Destain, J., et al. (2006). Journal of Molecular Catalysis B Enzymatic, 39, 117–123.

    Article  Google Scholar 

  15. Laemmli, U. K. (1970). Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  16. Conte-Júnior, C. A., Fernández, M., & Mano, S. B. (2006). A modern multidisciplinary applied microbiology: exploiting microbes and their interactions. New York: Wiley-VCH.

    Google Scholar 

  17. Rigo, E., Ninow, J. L., Polloni, A. E., Remonatto, D., Arbter, F., Vardanega, R., et al. (2009). Industrial Biotechnology, 5, 119–126.

    Article  CAS  Google Scholar 

  18. Jensen, R. G. (1983). Lipid, 18, 650–657.

    Article  CAS  Google Scholar 

  19. Barros, M., Fleuri, L. F., & Macedo, G. A. (2010). Brazilian Journal of Chemical Engineering, 27, 15–29.

    Article  CAS  Google Scholar 

  20. Chahinian, H., Ali, Y. B., Abousalham, A., Petry, S., Mandrich, L., Manco, G., et al. (2005). Biochimica et Biophysica Acta, 1738, 29–36.

    CAS  Google Scholar 

  21. Kim, J. T., Kang, S. G., Woo, J. H., Lee, J. H., Jeong, B. C., & Kim, S. J. (2007). Applied Microbiology and Biotechnology, 74, 820–828.

    Article  CAS  Google Scholar 

  22. Kojima, Y., & Shimizu, S. (2003). Journal of Bioscience and Bioengineering, 96, 219–226.

    CAS  Google Scholar 

  23. Colen, G., Junqueira, R. G., & Moraes-Santos, T. (2006). World Journal of Microbiology and Biotechnology, 22, 881–885.

    Article  CAS  Google Scholar 

  24. Castro-Ochoa, L. D., Rodríguez-Gómez, C., Valerio-Alfaro, G., & Oliart Ros, R. (2005). Enzyme and Microbial Technology, 37, 648–654.

    Article  CAS  Google Scholar 

  25. Wolski, E., Menusi, E., Remonatto, D., Vardanega, R., Arbter, F., Rigo, E., et al. (2009). LWT Food Science and Technology, 42, 1557–1560.

    Article  CAS  Google Scholar 

  26. Saxena, R. K., Davidson, W. S., Sheoran, A., & Giri, B. (2003). Process Biochemistry, 39, 239–247.

    Article  CAS  Google Scholar 

  27. Abbas, H., & Comeau, L. (2003). Enzyme and Microbial Technology, 32, 589–595.

    Article  CAS  Google Scholar 

  28. Langrand, G., Triantaphylides, C., & Baratti, J. (1988). Biotechnological Letters, 10, 549–554.

    Article  CAS  Google Scholar 

  29. Wang, D., Xu, Y., & Tianyu, S. (2008). Biochemical Engineering Journal, 41, 30–37.

    Article  Google Scholar 

  30. Gandhi, N. N. (1997). JAOCS, 74, 621–634.

    Article  CAS  Google Scholar 

  31. Gomes, F. M., Paula, A. V., Silva, G. S., & Castro, H. F. (2006). Quimica Nova, 29, 710–718.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank CNPq, CAPES, FAPERGS, and URI-Campus de Erechim for the financial support of this work and scholarships.

Author information

Authors and Affiliations

  1. Universidade Federal do Rio de Janeiro (UFRJ), Centro de Tecnologia, Av. Athos da Silveira Ramos, 149-Bloco A, Rio de Janeiro, 21941-909, Rio de Janeiro, Brazil

    Morgana Karin Pierozan & Enrique Guillermo Oestreicher

  2. Universidade Regional Integrada (URI), Campus de Erechim, Av. Sete de Setembro, Erechim, 1621-99700-000, Rio Grande do Sul, Brazil

    J. Vladimir Oliveira, Débora Oliveira, Helen Treichel & Rogério Luís Cansian

Authors
  1. Morgana Karin Pierozan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Enrique Guillermo Oestreicher
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Vladimir Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Débora Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Helen Treichel
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rogério Luís Cansian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Helen Treichel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pierozan, M.K., Oestreicher, E.G., Oliveira, J.V. et al. Studies on Immobilization and Partial Characterization of Lipases from Wheat Seeds (Triticum aestivum). Appl Biochem Biotechnol 165, 75–86 (2011). https://doi.org/10.1007/s12010-011-9234-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9234-y

Keywords

Search

Navigation