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Converting Carbohydrates Extracted from Marine Algae into Ethanol Using Various Ethanolic Escherichia coli Strains

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Abstract

Marine algae, which make up about 80% of the world’s living organisms, contain many energy sources, such as sugars and lipids. Therefore, the possibility of utilizing structural carbohydrates from marine algae for bioethanol production has been studied. In order to obtain monosaccharides, Undaria pinnatifida, Chlorella vulgaris, and Chlamydomonas reinhardtii were used for the saccharification experiments. The pretreatment was carried out by dilute acid hydrolysis and enzymatic treatment. To find the optimal conditions, experiments were performed at several temperatures, acid concentrations, pH conditions and durations. To test bioethanol production, several ethanolic E. coli W3110 strains, which were developed previously, were used. The maximum yield of bioethanol, 0.4 g ethanol/g biomass, was achieved with pretreated C. vulgaris and E. coli SJL2526, derived from wild-type E. coli W3110 and which includes the adhB, pdc, galP, and glk genes.

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Acknowledgments

This research was supported by a grant from Development of Marine-Bioenergy Program Funded by Ministry of Land, Transport and Maritime Affairs of Korean Government.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, South Korea

    Soojin Lee, Younghoon Oh, Donghyun Kim, Doyeon Kwon & Jinwon Lee

  2. Department of Biological Engineering, Inha University, Incheon, 402-751, South Korea

    Choulgyun Lee

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  1. Soojin Lee
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  2. Younghoon Oh
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  3. Donghyun Kim
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  4. Doyeon Kwon
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  5. Choulgyun Lee
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Correspondence to Jinwon Lee.

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Lee, S., Oh, Y., Kim, D. et al. Converting Carbohydrates Extracted from Marine Algae into Ethanol Using Various Ethanolic Escherichia coli Strains. Appl Biochem Biotechnol 164, 878–888 (2011). https://doi.org/10.1007/s12010-011-9181-7

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  • DOI: https://doi.org/10.1007/s12010-011-9181-7

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