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Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

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Abstract

Oxidative stress may produce high level of reactive oxygen species (ROS) following cell exposure to endogenous and exogenous factors. Recent experiments implicate oxidative stress as playing an essential role in cytotoxicity of many materials. The aim of this study was to measure intracellular malondialdehyde (MDA), advanced oxidation protein product (AOPP) levels, and superoxide dismutase (SOD) activities of L929 fibroblasts cultured on PDLLA, polyethylene glycol (PEG), or ethylenediamine (EDA) grafted PDLLA by plasma polymerization method. Cell proliferation on these scaffolds was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The study showed that MDA, AOPP levels, and SOD activities in L929 fibroblast cells cultured on all scaffolds were significantly different compared to the control group and each other. The highest MDA (0.42 ± 0.76 nmol/mg protein), AOPP (14.99 ± 4.67 nmol/mg protein) levels, and SOD activities (7.49 ± 3.74 U/mg protein) were observed in cells cultured on non-modified scaffolds; meanwhile, the most cell proliferation was obtained in EDA-modified scaffolds (MDA 0.15 ± 0.14 nmol/mg protein, AOPP 13.12 ± 3.86 nmol/mg protein, SOD 4.82 ± 2.64 U/mg protein). According to our finding, EDA- or PEG-modified scaffolds are potentially useful as suitable biomaterials in tissue engineering.

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Authors and Affiliations

  1. School of Health, Aksaray University, Aksaray, Turkey

    Melike Erol Demirbilek

  2. Nanotechnology and Nanomedicine Division, Hacettepe University, Beytepe, Ankara, Turkey

    Murat Demirbilek, Eda Yalçın & Emir Baki Denkbaş

  3. Department of Biology, Aksaray University, Aksaray, Turkey

    Zeynep Karahaliloğlu & Ebru Erdal

  4. Biochemistry Division, Department of Chemistry, Hacettepe University, Beytepe, Ankara, Turkey

    Tayfun Vural & Emir Baki Denkbaş

  5. Department of Secondary Science and Mathematics Education, Hacettepe University, Beytepe, Ankara, Turkey

    Necdet Sağlam

  6. Aksaray University, Aksaray, Turkey

    Necdet Sağlam

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  1. Melike Erol Demirbilek
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Correspondence to Melike Erol Demirbilek.

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Demirbilek, M.E., Demirbilek, M., Karahaliloğlu, Z. et al. Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds. Appl Biochem Biotechnol 164, 780–792 (2011). https://doi.org/10.1007/s12010-011-9173-7

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  • DOI: https://doi.org/10.1007/s12010-011-9173-7

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