Abstract
The main objective of this work is to present the optimization of the biotransformation of R-(+)-limonene and (−)-β-pinene aiming at the production of α-terpineol by strains of fungal and yeasts previously isolated by our research group using the methodology of experimental design. New optimized experimental data on α-terpineol production by the biotransformation of R-(+)-limonene and (−)-β-pinene using newly isolated microorganisms are reported in this work. Conversion of about 1,700 mg/L was achieved when R-(+)-limonene was used as substrate and the newly isolated strain 05.01.35 as microorganism at the central point of the experimental design, corresponding to a substrate concentration of 1.75%, mass of inoculum of 2 g, and substrate to ethanol volume ratio of 1:1. The same experimental condition led to higher conversions when (−)-β-pinene was used as substrates and the strains coded as 04.05.08 and 01.04.03 as microorganism. Here, conversions of about 770 mg/L were achieved.
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References
Adams, A., Demyttenaere, J. C. R., & De Kimpe, N. (2003). Food Chemistry, 80, 525–534.
Bessière, Y., & Thomas, A. F. (1989). Limonene. Natural Products Reports, 6(3), 291–309.
Bicas, J. L., Barros, F. F. C., Wagner, R., Godoy, H. T., & Pastore, G. M. (2008). Journal of Industrial Microbiology & Biotechnology, 35, 1061–1070.
Çelik, D., Bayraktar, E., & Mehmetoglu, Ü. (2004). Biochemical Engineering Journal, 17, 5–13.
Demyttenaere, J. C. R., Van Belleghem, K., & De Kimpe, N. (2001). Phytochemistry, 57, 199–208.
Farooq, A., Choudhary, M. I., Tahara, S., Rahman, A., Baser, K. H. C., & Demirci, F. (2002). Zeitschrift für Naturforschung, 57, 686–690.
Gershenzon, J., & Dudareva, N. (2007). Nature Chemical Biology, 3, 408–414.
Haaland, P. D. (1989). Experimental Design in Biotechnology. New York: Marcel Dekker.
Lindmark-Henriksson, M. (2003) Biotransformations of turpentine constituents: oxygenation and esterification. Doctoral Thesis. Mid Sweden University, Stockholm, Sweden, p 67
Manjarrez, N., Pére, H. I., Solís, A., Luna, H., Liévano, R., & Ramírez, M. (2007). Journal of the Brazilian Chemical Society, 18(4), 709–713.
Maróstica Junior, M. R., & Pastore, G. M. (2007). Food Chemistry, 101, 345–350.
Melo, L. L. M. M., Pastore, G. M., & Macedo, G. A. (2005). Process Biochemistry, 40, 3181–3185.
Onken, J., & Berger, R. G. (1999). Journal of Biotechnology, 69, 163–168.
Rottava, I., Cortina, P. F., Grando, C. E., Colla, A. R. S., Martelo, E., Cansian, R. L., et al. (2010). Applied Biochemistry and Biotechnology, 162, 719–732.
Rottava, I., Toniazzo, G., Cortina, P., Martello, E., Grando, C. E., Lerin, L. A., et al. (2010). LWT Food Science Technology, 43, 1128–1131.
Tan, Q., Day, D. F., & Cadwallader, K. R. (1998). Process Biochemistry, 33, 29–37.
Tecelão, C. S. R., van Keulen, F., & da Fonseca, M. M. R. (2001). Journal of Molecular Catalysis B Enzymatic, 11, 719–724.
Toniazzo, G., Lerin, L., Oliveira, D., Dariva, C., Cansian, R. L., Padilha, F. F., et al. (2006). Applied Biochemistry and Biotechnology, 132, 1023–1033.
van Dyk, M. S., van Resburg, E., & Moleleki, N. (1998). Biotechnological Letters, 20, 431–436.
van Resburg, E., Moleleki, N., van der, W. J. P., Botes, P. J., & van Dyk, M. S. (1997). Biotechnological Letters, 19, 779–782.
Yoo, S. K., & Day, D. F. (2002). Process Biochemistry, 37, 739–745.
Yoo, S. K., Day, D. F., & Cadwallader, A. (2001). Process Biochemistry, 36, 925–932.
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Rottava, I., Cortina, P.F., Martello, E. et al. Optimization of α-Terpineol Production by the Biotransformation of R-(+)-Limonene and (−)-β-Pinene. Appl Biochem Biotechnol 164, 514–523 (2011). https://doi.org/10.1007/s12010-010-9153-3
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DOI: https://doi.org/10.1007/s12010-010-9153-3