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Cell Surface Display of Cellulase Activity–Free Xylanase Enzyme on Saccharomyces Cerevisiae EBY100

Applied Biochemistry and Biotechnology volume 164, pages 294–304 (2011)Cite this article

Abstract

Cellulase-free xylanase has potential for its application in the selective removal of hemicellulose from kraft pulp to give good strength to paper. In this study, a gene (xyn) encoding cellulase activity–free xylanase enzyme (Xyn) was isolated from Paenibacillus polymyxa PPL-3. The xyn gene encoded a protein of 221 amino acids, and the purified Xyn was about 22.5 kDa measured by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Moreover, the cellulase activity–free xylanase enzyme (Xyn) was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using Aga2p as an anchor protein. Cell surface display of xylanase enzyme (Xyn) on S. cerevisiae EBY100 was confirmed by immunofluorescence microscopy. Optimum cell surface display of xylanase enzyme (Xyn) was observed at pH 7 and 40 °C. Therefore, cell surface–displayed xylanase enzyme (Xyn) can be used in the paper industry.

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Acknowledgements

This research was carried out by the financial support of Forest Services in 2010.

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Affiliations

  1. Department of Forest Products, IALS, Gyeongsang National University, Jinju, 660-701, South Korea

    Shabina Yeasmin, Chul Hawn Kim, Hyeon Jin Park, Mominul Islam Sheikh, Ji Yong Lee, Kyung Kil Back & Sung Ho Kim

  2. Department of Microbiology, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea

    Jae Won Kim

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  1. Shabina Yeasmin
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  2. Chul Hawn Kim
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  4. Mominul Islam Sheikh
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  7. Kyung Kil Back
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Correspondence to Chul Hawn Kim.

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Yeasmin, S., Kim, C.H., Park, H.J. et al. Cell Surface Display of Cellulase Activity–Free Xylanase Enzyme on Saccharomyces Cerevisiae EBY100. Appl Biochem Biotechnol 164, 294–304 (2011). https://doi.org/10.1007/s12010-010-9135-5

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  • DOI: https://doi.org/10.1007/s12010-010-9135-5

Keywords

  • Cellulase-free xylanase
  • Yeast surface display
  • Kraft pulp
  • Biobleaching
  • Paenibacillus polymyxa PPL-3