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Development of a Temperature Shift Strategy for Efficient Docosahexaenoic Acid Production by a Marine Fungoid Protist, Schizochytrium sp. HX-308

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Abstract

Temperature was one of the important environmental factors affecting the biosynthesis of docosahexaenoic acid (DHA; C22:6, ω−3). Generally, a low temperature would slow the strain growth, but promote the accumulation of unsaturated fatty acids. According to this information, the effects of temperature and different two-stage temperature shifting strategies on fatty acid production and DHA content of the marine fungoid protist, Schizochytrium sp. HX-308, were investigated in this study. Finally, the highest DHA percentage was up to 51.98% (per total fatty acids) with the DHA production of 6.05% (per dry cell weight), which was obtained with the method of shifting temperature from 30 °C for 32 h to 20 °C for 12 h.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Nos. 20936002 and 21006049), the National Basic Research Program of China (Nos. 2007CB707805, 2009CB724700 and 2011CB200906), the Scientific Research Project for Post-graduate in Jiangsu Province of China (No. CX07s_032z), the Fifth of Six Projects Sponsoring Talent Summits of Jiangsu Province (No. 2008-D-63), the Fok Ying Tung Education Foundation (No. 123014), the College Industrialization Project of Jiangsu Province (No. JH09-30), and the Program for New Century Excellent Talents in University from the Ministry of Education of China (No. NCET-09-0157).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People’s Republic of China

    Yan Zeng, Xiao-Jun Ji, Min Lian, Lu-Jing Ren, Li-Jing Jin, Ping-Kai Ouyang & He Huang

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  1. Yan Zeng
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  2. Xiao-Jun Ji
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  3. Min Lian
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  4. Lu-Jing Ren
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  6. Ping-Kai Ouyang
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  7. He Huang
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Correspondence to He Huang.

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Yan Zeng, Xiao-Jun Ji, and Min Lian contributed equally to this work.

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Zeng, Y., Ji, XJ., Lian, M. et al. Development of a Temperature Shift Strategy for Efficient Docosahexaenoic Acid Production by a Marine Fungoid Protist, Schizochytrium sp. HX-308. Appl Biochem Biotechnol 164, 249–255 (2011). https://doi.org/10.1007/s12010-010-9131-9

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