The genome of Dictyoglomus turgidum was sequenced and analyzed for carbohydrases. The broad range of carbohydrate substrate utilization is reflected in the high number of glycosyl hydrolases, 54, and the high percentage of CAZymes present in the genome, 3.09% of its total genes. Screening a random clone library generated from D. turgidum resulted in the discovery of five novel biomass-degrading enzymes with low homology to known molecules. Whole genome sequencing of the organism followed by bioinformatics-directed amplification of selected genes resulted in the recovery of seven additional novel enzyme molecules. Based on the analysis of the genome, D. turgidum does not appear to degrade cellulose using either conventional soluble enzymes or a cellulosomal degradation system. The types and quantities of glycosyl hydrolases and carbohydrate-binding modules present in the genome suggest that D. turgidum degrades cellulose via a mechanism similar to that used by Cytophaga hutchinsonii and Fibrobacter succinogenes.
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This work was funded in part by DOE grant DOE DE-FG36-06GO16106, “Novel enzyme products for the conversion of defatted soybean meal to ethanol,” and funded in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494). The work conducted by the U.S. Department of Energy Joint Genome Institute is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
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Brumm, P., Hermanson, S., Hochstein, B. et al. Mining Dictyoglomus turgidum for Enzymatically Active Carbohydrases. Appl Biochem Biotechnol 163, 205–214 (2011). https://doi.org/10.1007/s12010-010-9029-6
- Dictyoglomus turgidum
- Genome screening
- Thermophilic cellulase
- Enzyme discovery