Applied Biochemistry and Biotechnology

, Volume 162, Issue 8, pp 2381–2386

Enhancing Clostridial Acetone-Butanol-Ethanol (ABE) Production and Improving Fuel Properties of ABE-enriched Biodiesel by Extractive Fermentation with Biodiesel


DOI: 10.1007/s12010-010-9010-4

Cite this article as:
Li, Q., Cai, H., Hao, B. et al. Appl Biochem Biotechnol (2010) 162: 2381. doi:10.1007/s12010-010-9010-4


The extractive acetone–butanol–ethanol (ABE) fermentations of Clostridium acetobutylicum were evaluated using biodiesel as the in situ extractant. The biodiesel preferentially extracted butanol, minimized product inhibition, and increased production of butanol (from 11.6 to 16.5 gL−1) and total solvents (from 20.0 to 29.9 gL−1) by 42% and 50%, respectively. The fuel properties of the ABE-enriched biodiesel obtained from the extractive fermentations were analyzed. The key quality indicators of diesel fuel, such as the cetane number (increased from 48 to 54) and the cold filter plugging point (decreased from 5.8 to 0.2 °C), were significantly improved for the ABE-enriched biodiesel. Thus, the application of biodiesel as the extractant for ABE fermentation would increase ABE production, bypass the energy intensive butanol recovery process, and result in an ABE-enriched biodiesel with improved fuel properties.


Biobutanol Biodiesel Butanol inhibition Clostridium Extractant Low cold filter plugging point (CFPP) Solvent extractive fermentation 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.State Key Laboratory of Agricultural Microbiology, National Engineering Research Centre of Microbial Pesticides, College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.College of ScienceHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  3. 3.Department of Biological SciencesNorthern Illinois UniversityDeKalbUSA

Personalised recommendations