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Xylooligosaccharides Production from Alkali-Pretreated Sugarcane Bagasse Using Xylanases from Thermoascus aurantiacus

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Abstract

Sugarcane bagasse hemicellulose was isolated in a one-step chemical extraction using hydrogen peroxide in alkaline media. The polysaccharide containing 80.9% xylose and small amounts of l-arabinose, 4-O-methyl-d-glucuronic acid and glucose, was hydrolyzed by crude enzymatic extracts from Thermoascus aurantiacus at 50 °C. Conditions of enzymatic hydrolysis leading to the best yields of xylose and xylooligosaccharides (DP 2-5) were investigated using substrate concentration in the range 0.5–3.5% (w/v), enzyme load 40–80 U/g of the substrate, and reaction time from 3 to 96 h, applying a 22 factorial design. The maximum conversion to xylooligosaccharides (37.1%) was obtained with 2.6% of substrate and xylanase load of 60 U/g. The predicted maximum yield of xylobiose by a polynomial model was 41.6%. Crude enzymatic extract of T. aurantiacus generate from sugarcane bagasse hemicellulose 39% of xylose, 59% of xylobiose, and 2% of other xylooligosaccharides.

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Acknowledgments

The authors gratefully acknowledge the financial support of Fapesp and CNPq.

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Authors and Affiliations

  1. Department of Biotechnology, School of Engineering of Lorena, University of São Paulo—USP Estrada Municipal do Campinho, s/n°- CP 116, 12602-810, Lorena, SP, Brazil

    Michel Brienzo, Walter Carvalho & Adriane M. F. Milagres

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  1. Michel Brienzo
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  2. Walter Carvalho
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  3. Adriane M. F. Milagres
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Correspondence to Adriane M. F. Milagres.

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Brienzo, M., Carvalho, W. & Milagres, A.M.F. Xylooligosaccharides Production from Alkali-Pretreated Sugarcane Bagasse Using Xylanases from Thermoascus aurantiacus . Appl Biochem Biotechnol 162, 1195–1205 (2010). https://doi.org/10.1007/s12010-009-8892-5

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