Abstract
The aim of this study was the glycerolysis of babassu oil catalyzed by immobilized lipase from Burkholderia cepacia, in a continuous packed-bed reactor. The best reaction conditions were previously established in batchwise via response surface methodology as a function of glycerol-to-oil molar ratio and reaction temperature. The reactor operated continuously for 22 days at 50 °C, and during the first 6 days, no significant decrease on the initial lipase activity was observed. Monoglycerides concentration was in the range from 25 to 33 wt.%. Subsequently, a progressive decrease in the activity was detected, and an inactivation profile described by Arrhenius model estimated values of 50 days and 1.37 × 10−2 h−1, for the half-life and deactivation coefficient, respectively.
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Acknowledgements
The authors are grateful for the financial support provided by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), Brazil.
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de Freitas, L., dos Santos, J.C., Zanin, G.M. et al. Packed-Bed Reactor Running on Babassu Oil and Glycerol to Produce Monoglycerides by Enzymatic Route using Immobilized Burkholderia cepacia Lipase. Appl Biochem Biotechnol 161, 372–381 (2010). https://doi.org/10.1007/s12010-009-8858-7
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DOI: https://doi.org/10.1007/s12010-009-8858-7