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Packed-Bed Reactor Running on Babassu Oil and Glycerol to Produce Monoglycerides by Enzymatic Route using Immobilized Burkholderia cepacia Lipase

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Abstract

The aim of this study was the glycerolysis of babassu oil catalyzed by immobilized lipase from Burkholderia cepacia, in a continuous packed-bed reactor. The best reaction conditions were previously established in batchwise via response surface methodology as a function of glycerol-to-oil molar ratio and reaction temperature. The reactor operated continuously for 22 days at 50 °C, and during the first 6 days, no significant decrease on the initial lipase activity was observed. Monoglycerides concentration was in the range from 25 to 33 wt.%. Subsequently, a progressive decrease in the activity was detected, and an inactivation profile described by Arrhenius model estimated values of 50 days and 1.37 × 10−2 h−1, for the half-life and deactivation coefficient, respectively.

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Acknowledgements

The authors are grateful for the financial support provided by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), Brazil.

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Authors and Affiliations

  1. Engineering School of Lorena, University of São Paulo, P.O. Box 116-12602-810, Lorena, São Paulo, Brazil

    Larissa de Freitas, Julio C. dos Santos & Heizir F. de Castro

  2. Department of Chemical Engineering, State University of Maringa, Av. Colombo 5790, 46, 87020-900, Maringa-PR, Brazil

    Gisella Maria Zanin

Authors
  1. Larissa de Freitas
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  2. Julio C. dos Santos
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  3. Gisella Maria Zanin
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  4. Heizir F. de Castro
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Correspondence to Heizir F. de Castro.

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de Freitas, L., dos Santos, J.C., Zanin, G.M. et al. Packed-Bed Reactor Running on Babassu Oil and Glycerol to Produce Monoglycerides by Enzymatic Route using Immobilized Burkholderia cepacia Lipase. Appl Biochem Biotechnol 161, 372–381 (2010). https://doi.org/10.1007/s12010-009-8858-7

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  • DOI: https://doi.org/10.1007/s12010-009-8858-7

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