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Inhibition of Pro-inflammatory Secreted Phospholipase A2 by Extracts from Cynara cardunculus L.

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Abstract

The aim of the present work was to evaluate the anti-inflammatory properties of Cynara cardunculus L. (Asteraceae) during its growth using various solvents such as n-hexane, dichloromethane, acetone, and methanol for air-dried leaves and stems. The anti-inflammatory activities of crude extracts were evaluated by measuring the inhibition potency of mammalian non-pancreatic phospholipases A2 (hG-IIA). The methanol and acetone extracts of leaves harvested in February exhibit potent inhibition of hG-IIA (IC50 = 50 and 70 µg/ml, respectively). However, the acetone extract of stems harvested in December inhibits the hG-IIA with a lower IC50 around 130 µg/ml. Fractionation on silica gel and hydrophobic gel of the methanol extract of leaves harvested in February increases the inhibitory effect, and the IC50 reached 10 µg/ml.

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Acknowledgment

This work represents a part of Maher Kammoun thesis. We are grateful to Dr. Gérard Lambeau (Institute de Pharmacologie Moléculaire et Cellulaire CNRS-Sophia Antipolis, France) for his generous supply of the recombinant hG-IIA enzyme, for fruitful discussions, and for kind help during the preparation of this work. Our thanks are also addressed to Mr R. Hamrouni for correcting the English mistakes.

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Authors and Affiliations

  1. Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de soukra, BP1173-3038, Sfax, Tunisia

    Maher Kammoun, Yassine Ben Ali, Youssef Gargouri & Sofiane Bezzine

  2. Laboratoire de Chimie des Substances Naturelles, FSS, Sfax, Tunisia

    Imed Koubaa, Raoudha Jarraya & Mohamed Damak

Authors
  1. Maher Kammoun
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  2. Imed Koubaa
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  3. Yassine Ben Ali
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  5. Youssef Gargouri
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  7. Sofiane Bezzine
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Correspondence to Sofiane Bezzine.

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Kammoun, M., Koubaa, I., Ben Ali, Y. et al. Inhibition of Pro-inflammatory Secreted Phospholipase A2 by Extracts from Cynara cardunculus L.. Appl Biochem Biotechnol 162, 662–670 (2010). https://doi.org/10.1007/s12010-009-8849-8

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