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Simulated Microgravity Affects Growth of Escherichia coli and Recombinant β-d-Glucuronidase Production

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Abstract

Effects of simulated microgravity (SMG) on bacteria have been studied in various aspects. However, few reports are available about production of recombinant protein expressed by bacteria in SMG. In this study growth of E. coli BL21 (DE3) cells transformed with pET-28a (+)-pgus in double-axis clinostat that could model low shear SMG environment and the recombinant β-d-glucuronidase (PGUS) expression have been investigated. Results showed that the cell dry weights in SMG were 16.47%, 38.06%, and 28.79% more than normal gravity (NG) control, and the efficiency of the recombinant PGUS expression in SMG were 18.33%, 19.36%, and 33.42% higher than that in NG at 19 °C, 28 °C, and 37 °C, respectively (P < 0.05).

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Acknowledgments

This work was funded by the National “863” High-Tech Project (2008AA12A218-2), Natural Science Foundation of China (20776017), and the Natural Science Foundation of Beijing (5072028).

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Authors and Affiliations

  1. School of Life Science, Beijing Institute of Technology, South Zhongguancun Street, Beijing, 100081, People’s Republic of China

    Liang Xiang, Feng Qi, DaZhang Dai & Chun Li

  2. Center for Space and Applied Research Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China

    YuanDa Jiang

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  1. Liang Xiang
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  2. Feng Qi
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  3. DaZhang Dai
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  4. Chun Li
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  5. YuanDa Jiang
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Corresponding author

Correspondence to Chun Li.

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L. Xiang and F. Qi contributed equally to this work.

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Xiang, L., Qi, F., Dai, D. et al. Simulated Microgravity Affects Growth of Escherichia coli and Recombinant β-d-Glucuronidase Production. Appl Biochem Biotechnol 162, 654–661 (2010). https://doi.org/10.1007/s12010-009-8836-0

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  • DOI: https://doi.org/10.1007/s12010-009-8836-0

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