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Characterisation of Specific Activities and Hydrolytic Properties of Cell-Wall-Degrading Enzymes Produced by Trichoderma reesei Rut C30 on Different Carbon Sources

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Abstract

Conversion of lignocellulosic substrates is limited by several factors, in terms of both the enzymes and the substrates. Better understanding of the hydrolysis mechanisms and the factors determining their performance is crucial for commercial lignocelluloses-based processes. Enzymes produced on various carbon sources (Solka Floc 200, lactose and steam-pre-treated corn stover) by Trichoderma reesei Rut C30 were characterised by their enzyme profile and hydrolytic performance. The results showed that there was a clear correlation between the secreted amount of xylanase and mannanase enzymes and that their production was induced by the presence of xylan in the carbon source. Co-secretion of α-arabinosidase and α-galactosidase was also observed. Secretion of β-glucosidase was found to be clearly dependent on the composition of the carbon source, and in the case of lactose, 2-fold higher specific activity was observed compared to Solka Floc and steam-pre-treated corn stover. Hydrolysis experiments showed a clear connection between glucan and xylan conversion and highlighted the importance of β-glucosidase and xylanase activities. When hydrolysis was performed using additional purified β-glucosidase and xylanase, the addition of β-glucosidase was found to significantly improve both the xylan and glucan conversion.

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Acknowledgements

The European Cooperation in the field of Scientific and Technical Research (COST Action FP0602. Biotechnology for Lignocellulose Biorefineries) is acknowledged for mobility support. The Hungarian National Research Fund (OTKA – K 72710) is kindly acknowledged for financial support. Celluclast 1.5L and Novozym 188 were kindly donated by Novozymes A/S (Bagsvaerd. Denmark). Steam-pre-treated corn stover and steam-pre-treated spruce were received from ENEA, Italy and Lund University, Sweden, respectively. Ulla Vornamo is gratefully acknowledged for her help during the experiments.

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Authors and Affiliations

  1. Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Szt. Gellért tér 4, 1111, Budapest, Hungary

    Bálint Sipos, Zsuzsa Benkő, Dóra Dienes & Kati Réczey

  2. Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    Liisa Viikari

  3. VTT Biotechnology, Tietotie 2, 02150, Espoo, Finland

    Matti Siika-aho

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  1. Bálint Sipos
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  2. Zsuzsa Benkő
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Correspondence to Bálint Sipos.

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Sipos, B., Benkő, Z., Dienes, D. et al. Characterisation of Specific Activities and Hydrolytic Properties of Cell-Wall-Degrading Enzymes Produced by Trichoderma reesei Rut C30 on Different Carbon Sources. Appl Biochem Biotechnol 161, 347–364 (2010). https://doi.org/10.1007/s12010-009-8824-4

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