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Production and Characterization of a Novel Laccase with Cold Adaptation and High Thermal Stability from an Isolated Fungus

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Abstract

A new white-rot fungus SYBC-L1, which could produce an extracellular laccase, was isolated from a decayed Elaeocarpus sylvestris. The strain was identified as Pycnoporus sp. SYBC-L1 according to the morphological characteristics and ribosomal ITS1-5.8S-ITS2 RNA genomic sequence analysis. The highest laccase activity of 24.1 U ml−1, which was approximately 40-fold than that in basal medium, was achieved in optimal culture medium in submerged fermentation. The laccase produced by Pycnoporus sp. SYBC-L1 was not only a cold adaptation enzyme with a relative catalytic activity of 30.2% at 0°C but also a high thermostable enzyme. The half-lives at 60, 70 and 80°C were 85.5, 37.2, and 2.6 h, respectively. The laccase could effectively decolorize weak acid blue AS and diamond black PV up to 88% and 74.7%, respectively, within 2 h in the absence of any redox mediators. The results suggested Pycnoporus sp. SYBC-L1 was a potential candidate for laccase production and industrial application.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Zhixin Wang, Yujie Cai, Xiangru Liao & Feng Zhang

  2. Jiangsu Hanbon Science & Technology Co. Ltd, Huai’an, Jiangsu, 223001, People’s Republic of China

    Dabing Zhang & Zhiling Li

Authors
  1. Zhixin Wang
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  2. Yujie Cai
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  3. Xiangru Liao
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  4. Feng Zhang
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  5. Dabing Zhang
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  6. Zhiling Li
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Correspondence to Yujie Cai or Xiangru Liao.

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Wang, Z., Cai, Y., Liao, X. et al. Production and Characterization of a Novel Laccase with Cold Adaptation and High Thermal Stability from an Isolated Fungus. Appl Biochem Biotechnol 162, 280–294 (2010). https://doi.org/10.1007/s12010-009-8801-y

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  • DOI: https://doi.org/10.1007/s12010-009-8801-y

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