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Synthesis of Rapeseed Biodiesel Using Short-Chained Alkyl Acetates as Acyl Acceptor

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Abstract

In this study, we conducted experiments using a response surface methodology to determine the optimal reaction conditions for the enzymatic synthesis of biodiesel from rapeseed oil and short-chained alkyl acetates, such as methyl acetate or ethyl acetate, as the acyl acceptor at 40 °C. Based on our response surface methodology experiments, the optimal reaction conditions for the synthesis of biodiesel were as follows: methyl acetate as acyl acceptor, catalyst concentration of 16.50%, oil-to-methyl acetate molar ratio of 1:12.44, and reaction time of 19.70 h; ethyl acetate as acyl acceptor, catalyst concentration of 16.95%, oil-to-ethyl acetate molar ratio of 1:12.56, and reaction time of 19.73 h. The fatty acid ester content under the above conditions when methyl acetate and ethyl acetate were used as the acyl acceptor was 58.0% and 62.6%, respectively. The statistical method described in this study can be applied to effectively optimize the enzymatic conditions required for biodiesel production with short-chained alkyl acetates.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (KRF-2008-313-D00303). And this work is partially the outcome of the fostering project of the Specialized Graduate School, which is financially supported by the Ministry of Knowledge Economy.

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Authors and Affiliations

  1. Department of Biotechnology, Pukyong National University, Busan, 608-737, Korea

    Gwi-Taek Jeong

  2. School of Biological Sciences and Technology, Interdisciplinary Program of Graduate School for Bioenergy & Biomaterials, Research Institute for Catalysis, Functional Food Research Center, Biotechnology Research Institute, Chonnam National University, Gwangju, 500-757, Korea

    Don-Hee Park

  3. School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Korea

    Don-Hee Park

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  1. Gwi-Taek Jeong
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  2. Don-Hee Park
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Correspondence to Don-Hee Park.

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Jeong, GT., Park, DH. Synthesis of Rapeseed Biodiesel Using Short-Chained Alkyl Acetates as Acyl Acceptor. Appl Biochem Biotechnol 161, 195–208 (2010). https://doi.org/10.1007/s12010-009-8777-7

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  • DOI: https://doi.org/10.1007/s12010-009-8777-7

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