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Response Surface Optimization of Medium Components for Naringinase Production from Staphylococcus xylosus MAK2

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Abstract

Response surface methodology was used to optimize the fermentation medium for enhancing naringinase production by Staphylococcus xylosus. The first step of this process involved the individual adjustment and optimization of various medium components at shake flask level. Sources of carbon (sucrose) and nitrogen (sodium nitrate), as well as an inducer (naringin) and pH levels were all found to be the important factors significantly affecting naringinase production. In the second step, a 22 full factorial central composite design was applied to determine the optimal levels of each of the significant variables. A second-order polynomial was derived by multiple regression analysis on the experimental data. Using this methodology, the optimum values for the critical components were obtained as follows: sucrose, 10.0%; sodium nitrate, 10.0%; pH 5.6; biomass concentration, 1.58%; and naringin, 0.50% (w/v), respectively. Under optimal conditions, the experimental naringinase production was 8.45 U/mL. The determination coefficients (R 2) were 0.9908 and 0.9950 for naringinase activity and biomass production, respectively, indicating an adequate degree of reliability in the model.

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Acknowledgement

The authors would like to thank the Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing financial assistance to carry out this study from a project grant CSIR 38(1133)/07/ EMR-II. Aneet Kaur gratefully acknowledges the award of SRF (Senior Research Fellowship).

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Authors and Affiliations

  1. Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147 002, India

    Munish Puri, Aneet Kaur, Ram Sarup Singh & Anubhav Singh

  2. Centre for Biotechnology and Interdisciplinary Sciences (Biodeakin), Institute for Technology Research and Innovation (ITRI), Deakin University, Victoria, 3217, Australia

    Munish Puri

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  1. Munish Puri
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  2. Aneet Kaur
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Correspondence to Munish Puri.

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Puri, M., Kaur, A., Singh, R.S. et al. Response Surface Optimization of Medium Components for Naringinase Production from Staphylococcus xylosus MAK2 . Appl Biochem Biotechnol 162, 181–191 (2010). https://doi.org/10.1007/s12010-009-8765-y

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  • DOI: https://doi.org/10.1007/s12010-009-8765-y

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