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Influence of Extruder Temperature and Screw Speed on Pretreatment of Corn Stover while Varying Enzymes and Their Ratios

Applied Biochemistry and Biotechnology volume 162, pages 264–279 (2010)Cite this article

Abstract

Pretreatment is being the first and most expensive step, it has pervasive impacts on all other steps in overall conversion process. There are several pretreatment methods using physical, chemical, and biological principles which are under various stages of investigation. Extrusion can be used as one of the physical pretreatment methods towards biofuel production. The objective of this study was to evaluate the effect of barrel temperature and screw speed on sugar recovery from corn stover, to select a suitable enzyme combination and its ratio. Corn stover was pretreated in a single screw extruder with five screw speeds (25, 50, 75, 100, and 125 rpm) and five barrel temperatures (25, 50, 75, 100, and 125 °C). In order to select a suitable enzyme combination and ratio, different levels of cellulase and β-glucosidase, multienzyme complex and β-glucosidase were used during saccharification of pretreated corn stover. From the statistical analysis, it was found that screw speed and temperature had a significant effect on sugar recovery from corn stover. Higher glucose, xylose, and combined sugar recovery of 75, 49, and 61%, respectively, were recorded at 75 rpm and 125 °C. This pretreatment condition resulted in 2.0, 1.7, and 2.0 times higher than the control sample using 1:4 cellulase and β-glucosidase combination.

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Acknowledgments

This research was supported by funding from the Agricultural Experiment Station and North Central Sun Grant Center at South Dakota State University through a grant provided by the US Department of Transportation, Office of the Secretary, Grant No. DTOS59-07-G-00054. Also, enzymes supplied by Novozymes, Inc for conducting this study were greatly appreciated.

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  1. Agricultural & Biosystems Engineering Department, South Dakota State University, Brookings, SD, 57007, USA

    Chinnadurai Karunanithy & Kasiviswanathan Muthukumarappan

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  1. Chinnadurai Karunanithy
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  2. Kasiviswanathan Muthukumarappan
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Correspondence to Chinnadurai Karunanithy.

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Karunanithy, C., Muthukumarappan, K. Influence of Extruder Temperature and Screw Speed on Pretreatment of Corn Stover while Varying Enzymes and Their Ratios. Appl Biochem Biotechnol 162, 264–279 (2010). https://doi.org/10.1007/s12010-009-8757-y

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  • DOI: https://doi.org/10.1007/s12010-009-8757-y

Keywords

  • Biomass
  • Pretreatment
  • Extrusion
  • Screw speed
  • Temperature
  • Enzymatic hydrolysis
  • Sugar recovery