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Maximization of β-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects

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Abstract

In this work, the agitation and aeration effects in the maximization of the β-galactosidase production from Kluyveromyces marxianus CCT 7082 were investigated simultaneously, in relation to the volumetric enzyme activity and the productivity, as well as the analysis of the lactose consumption and production of glucose, and galactose of this process. Agitation and aeration effects were studied in a 2 L batch stirred reactor. A central composite design (22 trials plus three central points) was carried out. Agitation speed varied from 200 to 500 rpm and aeration rate from 0.5 to 1.5 vvm. It has been shown in this study that the volumetric enzyme production was strongly influenced by mixing conditions, while aeration was shown to be less significant. Linear models for activity and productivity due to agitation and aeration were obtained. The favorable condition was 500 rpm and 1.5 vvm, which lead to the best production of 17 U mL−1 for enzymatic activity, 1.2 U mL−1 h−1 for productivity in 14 h of process, a cellular concentration of 11 mg mL−1, and a 167.2 h−1 volumetric oxygen transfer coefficient.

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Abbreviations

K L a :

Volumetric oxygen transfer coefficient (h−1)

rpm:

Revolution per minute

v/v:

Inoculum/broth volume

vvm:

Air volume per broth volume per minute

Y X/S :

Cell yield on substrate

Y P/X :

Product yield on substrate

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Acknowledgements

The authors would like to thank CAPES/PROCAD for the financial support.

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Authors and Affiliations

  1. Chemistry and Food School, Federal University of Rio Grande (FURG), CP 474, CEP 96201-900, Rio Grande, Rio Grande do Sul, Brazil

    Fernanda Germano Alves, Janaína Fernandes de Medeiros Burkert & Susana Juliano Kalil

  2. Department of Food Engineering, University of Campinas (UNICAMP), CP 6121, CEP 13083-970, Campinas, São Paulo, Brazil

    Francisco Maugeri Filho

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  1. Fernanda Germano Alves
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Correspondence to Susana Juliano Kalil.

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Alves, F.G., Filho, F.M., de Medeiros Burkert, J.F. et al. Maximization of β-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects. Appl Biochem Biotechnol 160, 1528–1539 (2010). https://doi.org/10.1007/s12010-009-8683-z

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