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Biotransformation of Paeonol and Emodin by Transgenic Crown Galls of Panax quinquefolium

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Abstract

Two aromatic substrates, paeonol (1) and emodin (2), were biotransformed by using transgenic crown galls of Panax quinquefolium. Four biotransformed products (3–6) were isolated and identified by physicochemical and spectral methods. A β-glucoside (3, 73.2% of biotransformation yield) and a 1-(2,4-dimethoxyphenyl)- ethanone (4, 8.03%) were isolated from the suspension cultures after 7-day incubation of substrate 1. Upon administration of substrate 2, another β-glucoside [emodin-6-O-β-d-glucopyranoside (5), 19.2%] and a hydroxylated derivative, citreorosein (6, 54.6%), were also obtained. The results demonstrate that transgenic crown galls of P. quinquefolium have the capacities to catalyze glycosylation, hydroxylation, and methylation reactions in the plant cells on those aromatic compounds.

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Abbreviations

UDP:

Uridine bisphosphate

MS Medium:

Murashige and Skoog Medium (1962)

TLC:

Thin-layer chromatography

Varian Inova NMR:

Varian Inova nuclear magnetic resonance

TMS:

Tetramethylsilane

HPLC:

High-performance liquid chromatography

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Acknowledgement

This research work was financially supported by the Fund for Key Subjects of Ministry of Education of China (no. 104180) and Natural Sciences Fund of Guangdong (no. 31891). The authors thank Dongbo Yu at University of Texas Southwestern Medical Center, USA, for proof-reading our manuscript.

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Authors and Affiliations

  1. College of Pharmacy, Jinan University, Guangzhou, 510632, China

    W. L. Ma, C. Y. Yan, J. H. Zhu, G. Y. Duan & R. M. Yu

  2. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    C. Y. Yan

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  1. W. L. Ma
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  2. C. Y. Yan
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  3. J. H. Zhu
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  4. G. Y. Duan
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Correspondence to R. M. Yu.

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Ma, W.L., Yan, C.Y., Zhu, J.H. et al. Biotransformation of Paeonol and Emodin by Transgenic Crown Galls of Panax quinquefolium . Appl Biochem Biotechnol 160, 1301–1308 (2010). https://doi.org/10.1007/s12010-009-8655-3

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