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Role of Toll-Like Receptor 3, RIG-I, and MDA5 in the Expression of Mesothelial IL-8 Induced by Viral RNA

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Abstract

Interleukin-8 (IL-8) is a chemokine that has been shown to be a potent chemoattractant for polymorphonuclear neutrophils from the vascular compartment into the pleural space during infectious pleural effusions. Mesothelial cells express the viral receptors Toll-like receptor 3 (TLR3), RIG-I, and MDA5. Activation of these receptors by viral RNA exemplified by poly (I:C) RNA leads to a time- and dose-dependent increase of mesothelial IL-8 synthesis. To show the specific effect of viral receptors, knockdown experiments with short interfering RNA specific for TLR3, RIG-I and MDA5 were performed. This novel finding of functional expression of these viral sensors on human mesothelial cells may indicate a novel link between viral infections and mesothelial inflammation and indicates a pathophysiologic role of viral receptors in these processes.

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Authors and Affiliations

  1. Medical Policlinic, Ludwig-Maximilians-University, Pettenkoferstrasse 8a, 80336, Munich, Germany

    Markus Wörnle, Matthias Sauter, Kathrin Kastenmüller, Andrea Ribeiro & Thomas Sitter

  2. Department of Surgery, Ludwig-Maximilians-University, Munich, Germany

    Thomas Mussack & Roland Ladurner

Authors
  1. Markus Wörnle
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  2. Matthias Sauter
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  3. Kathrin Kastenmüller
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  4. Andrea Ribeiro
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  5. Thomas Mussack
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  7. Thomas Sitter
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Correspondence to Markus Wörnle.

Additional information

This work was supported by a grant from the Else-Kröner-Fresenius-Stiftung to Matthias Sauter and Thomas Sitter and from the Deutsche Nierenstiftung to Markus Wörnle.

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Wörnle, M., Sauter, M., Kastenmüller, K. et al. Role of Toll-Like Receptor 3, RIG-I, and MDA5 in the Expression of Mesothelial IL-8 Induced by Viral RNA. Appl Biochem Biotechnol 160, 1179–1187 (2010). https://doi.org/10.1007/s12010-009-8643-7

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  • DOI: https://doi.org/10.1007/s12010-009-8643-7

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