Abstract
Alcaligenes sp. ECU0401 has been isolated from soil samples with high nitrilase activity against glycolonitrile using the enrichment culture technique. The preferred carbon/nitrogen sources and metal ions were sodium acetate, a composite of peptone and yeast extract, and Cu2+, respectively. Glycolic acid was obtained in a yield of 96.5% after 14 h of biotransformation from a total of 200 mM glycolonitrile in the mode of sequential addition during the cultivation of Alcaligenes sp. ECU0401 in a 5-L jar fermenter. Fifty micromolars of glycolonitrile could be hydrolyzed in a yield of 94.1% by resting cells after 36 h. The microbial nitrilase system could hydrolyze various nitriles with high activity, and no amidase activity and glycolic acid were observed in hydrolyzing glycolamide. It significantly exhibited high enantioselectivity in the hydrolysis of mandelonitrile and 2-chloromandelonitrile (>99.9% e.e. p ). Efficient biocatalyst recycling was achieved as a result of immobilization in glutaraldehyde/polyethylenimine cross-linked carrageenan with immobilized cells exhibiting a biocatalyst productivity of 1,042.2 g glycolic acid per gram dry cell weight after 29 batch recycles.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Liang, L. Y., Zheng, Y. G., & Shen, Y. C. (2008). Process Biochemistry, 43(7), 758–764. doi:10.1016/j.procbio.2008.03.002.
Chen, J., Zheng, Y. G., & Shen, Y. C. (2008). Process Biochemistry, 43(9), 978–983. doi:10.1016/j.procbio.2008.05.002.
Wang, M. X. (2005). Topics in Catalysis, 35, 117–130. doi:10.1007/s11244-005-3817-1.
Asano, Y., Tani, Y., & Yamada, H. (1980). Agricultural and Biological Chemistry, 44, 2251–2252.
Zhu, D., Mukherjee, C., Yang, Y., Rios, B. E., Gallagher, D. T., Smith, N. N., et al. (2008). Journal of Biotechnology, 133, 327–333. doi:10.1016/j.jbiotec.2007.10.001.
Shi, Y. H., Sun, H. Y., Lu, D. M., Le, Q. H., Chen, D. X., & Zhou, Y. C. (2006). Separation and Purification Technology, 49(1), 20–26. doi:10.1016/j.seppur.2005.08.002.
DiCosimo, R., Payne, M.S., Panova, A., Thompson, J., & O'Keefe, D.P.(2007). U.S. Patent, 7,198,927.
Ebmeyer, F., Haberlein, H., Haberlein, H.H., Haberlein, J.T., Haberlein, M.C., & Mohn, H.(1998). U.S. Patent, 5,723,662.
Hu, J. G., Wang, Y. J., Zheng, Y. G., & Shen, Y. C. (2007). Enzyme and Microbial Technology, 41, 244–249. doi:10.1016/j.enzmictec.2007.01.014.
Gavagan, J.E., Chauhan, S., DiCosimo, R., Fallon, R.D., & Gavagan, J.E.Payne, & M.S. (2002). U.S. Patent. USP 6,416,980.
Xue, J.P., Luo, J.X., Li, H.B., Yu, J.H., Zhu, J., Xu, H., & Shen, Y.C.(2006). Chinese Patent, CN1772912.
Tamura, K. (1996). European Patent, EP0,711,836A1.
Yamaguchi, Y., Ushigome, M., & Kato, T.(1998). U.S. Patent, 5,756,306.
Panova, A., Mersinger, L. J., Liu, Q., Foo, T., Roe, D. C., Spillan, W. L., et al. (2007). Advanced Synthesis & Catalysis, 349(8-9), 1462–1474. doi:10.1002/adsc.200700061.
Ben-Bassat, A., Walls, A. M., Plummer, M. A., Sigmund, A. E., Spillan, W. L., & DiCosimo, R. (2008). Advanced Synthesis & Catalysis, 350(11-12), 1761–1769. doi:10.1002/adsc.200800228.
Wu, S. J., Fogiel, A. J., Petrillo, K. L., Jackson, R. E., Parker, K. N., Parker, K. N., et al. (2008). Biotechnology and Bioengineering, 99(3), 717–720. doi:10.1002/bit.21643.
He, Y. C., Xu, J. H., Xu, Y., Ouyang, L. M., & Pan, J. (2007). Chinese Chemical Letters, 18, 677–680. doi:10.1016/j.cclet.2007.04.034.
Chen, S. P., Huang, T., & Sun, S. G. (2005). Journal of Chromatography. A, 1089, 142–147. doi:10.1016/j.chroma.2005.06.076.
Fawcett, J. K., & Scott, J. E. (1960). Journal of Clinical Pathology, 13, 156–159. doi:10.1136/jcp. 13.2.156.
Weisburg, W. G., Barns, S. M., Pelletier, D. A., & Lane, D. J. (1991). 16 S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173, 697–703.
Zhang, Z. J., Xu, J. H., & He, Y. C. (2008). 12th Asia Pacific Confederation of Chemical Engineering Congress, 4, 232.
Kobayashi, M., Izui, H., Nagasawa, T., & Yamada, H. (1993). Proceedings of the National Academy of Sciences of the United States of America, 90, 247–251. doi:10.1073/pnas.90.1.247.
Nagasawa, T., Kobayashi, M., & Yamada, H. (1998). Archives of Microbiology, 150, 89–94. doi:10.1007/BF00409723.
Naik, S. C., Kaul, P., Barse, B., Banerjee, A., & Banerjee, U. C. (2008). Bioresource Technology, 99, 26–31. doi:10.1016/j.biortech.2006.11.053.
Banerjee, A., Kaul, P., & Banerjee, U. C. (2006). Applied Microbiology and Biotechnology, 72, 77–87. doi:10.1007/s00253-005-0255-8.
Nagasawa, T., Nakamura, T., & Yamada, H. (1990). Archives of Microbiology, 155, 13–17. doi:10.1007/BF00291267.
Yamamoto, K., Oishi, K., Fujimatsu, I., & Komatsu, K. I. (1991). Applied and Environmental Microbiology, 57, 3028–3032.
Nagasawa, T., Mauger, J., & Yamada, H. (1990). European Journal of Biochemistry, 194, 765–772. doi:10.1111/j.1432-1033.1990.tb19467.x.
Kobayashi, M., Yanaka, N., Nagasawa, T., & Yamada, H. (1990). Journal of Bacteriology, 172(9), 4807–4815.
He, Y. C., Xu, J. H., Pan, J., Ouyang, L. M., & Xu, Y. (2008). Bioprocess and Biosystems Engineering, 31(5), 445–451. doi:10.1007/s00449-007-0181-5.
Acknowledgments
This work was financially supported by the National Natural Science Foundation of China (nos. 20506037 and 20672037), the Ministry of Science and Technology (no. 2007AA02Z225), and the Open Project Program of the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (no. 2008004). Cordial thanks are given to Dr. Yunhai Shi at ECUST for his kind donation of glycolonitrile.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
He, YC., Xu, JH., Su, JH. et al. Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401. Appl Biochem Biotechnol 160, 1428–1440 (2010). https://doi.org/10.1007/s12010-009-8607-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-009-8607-y