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Aerobic Biodegradation Pathway for Remazol Orange by Pseudomonas aeruginosa

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Abstract

Removal of azo dyes from effluent generated by textile industries is rather difficult. Azo dyes represent a major class of synthetic colorants that are mutagenic and carcinogenic. Pseudomonas aeruginosa grew well in the presence of Remazol Orange (RO) and was able to decolorize and degrade it. In the present study, the decolorization and degradation efficiency using single culture P. aeruginosa with RO and textile wastewaters is studied. The elucidation of decolorization pathway for P. aeruginosa is of special interest. The degradation pathway and the metabolic products formed during the degradation were also predicted with the help of high performance liquid chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy analysis. The data show the cleavage of the azo dye RO to form both methyl metanilic acid and 4-aminobenzoic acid after decolorization and finally to oxidation forms benzoic acid, alkenes, aldehydes, and alkynes. The organism was able to decolorize the dye RO and wastewater effectively to the maximum of 82.4% and 62%, respectively.

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Acknowledgments

We thank the director of the National Environmental Engineering Research Institute, Nagpur, India for allowing us to publish this work. We are grateful to DBT, New Delhi for funding the project.

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  1. National Environmental Engineering Research Institute, CSIR Complex, Chennai, 600113, India

    K. Sarayu & S. Sandhya

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  1. K. Sarayu
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  2. S. Sandhya
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Correspondence to S. Sandhya.

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Sarayu, K., Sandhya, S. Aerobic Biodegradation Pathway for Remazol Orange by Pseudomonas aeruginosa . Appl Biochem Biotechnol 160, 1241–1253 (2010). https://doi.org/10.1007/s12010-009-8592-1

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