Abstract
Gln49-PLA2 is a phospholipase A2 isolated from the Gloydius ussurensis snake venom. In this paper, we studied its effect on the function of neural conduction. Electrophysiological studies demonstrated that Gln49-PLA2 reduced the amplitude of the action potential and the velocity of nerve conduction on isolated mouse sciatic nerve. Patch clamp recordings confirmed that Gln49-PLA2 significantly decreased neural excitability by the potentiation of sodium channels and the blockade of potassium channels in nerve terminal. In freshly isolated hippocampal pyramidal neurons, 54.25% of potassium current was inhibited by 20 μg/ml Gln49-PLA2. However, sodium current was potentiated by 158.99% under the same condition. These findings demonstrate that the effect of Gln49-PLA2 on ion channels is the main mechanism of analgesic action.
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Abbreviations
- PLA2 :
-
phospholipase A2
- Gln49-PLA2 :
-
phospholipase A2 with Gln at position 49 from Gloydius ussurensis
- HEPES:
-
N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid
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Acknowledgments
This work was supported by grants from Liaoning Department of Science and Technology. We thank Professor Changsen Sun from the school of physics and optoelectronic technology, Dalian University of Technology for the ion channel current assay. We also thank Qiling Song from the Department of Fine Chemical Technology, Dalian University of Technology for the action potential and nerve conduction velocity assay.
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Zhang, Y., Jiang, B., Li, W. et al. Mechanisms of Analgesic Action of Gln49-PLA2 from Gloydius ussurensis Snake Venom. Appl Biochem Biotechnol 160, 773–779 (2010). https://doi.org/10.1007/s12010-009-8573-4
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DOI: https://doi.org/10.1007/s12010-009-8573-4