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Dried–Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems

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Abstract

New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5–12.5 °C and 15–65 °C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92–93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.

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Abbreviations

OPH:

Organophosphorus hydrolase

polyHis:

Polyhistidine sequence

OPC:

Organophosphorous compound

IDA-cryoPAAG:

Polyacrylamide cryogel modified by iminodiacetic acid residues

N-His6-OPH/OPH-His6-C:

OPH with N-/C-terminal hexahistidine tag

N-His12-OPH:

OPH with N-terminal dodecahistidine tag

i-N-His6-OPH/i-OPH-His6-C/i-N-His12-OPH:

Immobilized N-His6-OPH/OPH-His6-C/N-His12-OPH

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Acknowledgments

This research was financially supported by the Federal Agency for Science and Innovation of Russian Federation (Contract No. 02.515.11.5002) and Russian Foundation for Basic Research (grant nos. 07-04-12025-ofi and 08-04-12050-ofi).

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Authors and Affiliations

  1. Chemical Enzymology Department, Chemistry Faculty, The M.V. Lomonosov Moscow State University, Lenin’s Hills 1/11, Moscow, 119992, Russia

    Elena N. Efremenko & Ilya V. Lyagin

  2. Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 22100, Lund, Sweden

    Fatima M. Plieva, Igor Yu. Galaev & Bo Mattiasson

  3. Protista Biotechnology AB, IDEON, 22370, Lund, Sweden

    Fatima M. Plieva

Authors
  1. Elena N. Efremenko
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  2. Ilya V. Lyagin
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  3. Fatima M. Plieva
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  4. Igor Yu. Galaev
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  5. Bo Mattiasson
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Correspondence to Elena N. Efremenko.

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Efremenko, E.N., Lyagin, I.V., Plieva, F.M. et al. Dried–Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems. Appl Biochem Biotechnol 159, 251–260 (2009). https://doi.org/10.1007/s12010-009-8564-5

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