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Stagewise Dilute-Acid Pretreatment and Enzyme Hydrolysis of Distillers’ Grains and Corn Fiber

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Abstract

Distillers’ grains and corn fiber are the coproducts of the corn dry grind and wet milling industries, respectively. Availability of distillers’ grains and corn fiber at the ethanol plant and their high levels of lignocellulosic material make these coproducts attractive feedstocks for conversion to ethanol. In this study, dilute sulfuric acid hydrolysis of these coproducts was investigated in a multistage scheme. After the completion of each pretreatment stage, the liquid substrate was separated and reused in the succeeding pretreatment stage with a fresh substrate. The substrate from each stage was also subjected to enzyme hydrolysis in a separate experiment. The sulfuric acid concentration and the substrate loading were maintained at 1.0 vol% and 15.0 wt.%, respectively, and the temperature was maintained at 120 °C in all the experiments. Experiments were also performed to study the effect of removing oil from the samples prior to the pretreatment. The highest concentration of monomeric sugars (MS) was observed when three stages of pretreatment were followed by the enzyme reaction. The enzyme hydrolysis of the three-stage pretreated dried distillers’ grains and corn fiber yielded 122.6 ± 5.8 and 184.5 ± 4.1 mg/mL of MS, respectively. The formation of inhibitory products was also monitored.

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Acknowledgments

The authors express their gratitude to the Nebraska Research Initiative for their support of this work.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, 207H Othmer Hall, Lincoln, NE, 68588-0643, USA

    Hossein Noureddini, Jongwon Byun & Ta-Jen Yu

Authors
  1. Hossein Noureddini
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  2. Jongwon Byun
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  3. Ta-Jen Yu
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Correspondence to Hossein Noureddini.

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Noureddini, H., Byun, J. & Yu, TJ. Stagewise Dilute-Acid Pretreatment and Enzyme Hydrolysis of Distillers’ Grains and Corn Fiber. Appl Biochem Biotechnol 159, 553–567 (2009). https://doi.org/10.1007/s12010-009-8544-9

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