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Colloidal Nanogold-Based Immunochromatographic Strip Test for the Detection of Digoxin Toxicity

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Abstract

Digoxin is widely used as a cardiac glycoside drug in the treatment of various heart conditions. Because it is a toxic drug, it should be regularly monitored in the serum of patients under treatment. In this study, colloidal nanogold is synthesized and the preparation of nanogold-labeled monoclonal antibody probe to digoxin is described under optimal conditions. In addition, an immunochromatographic (IC) method for digoxin analysis employing nanogold-labeled probe is developed. With this technique, it requires only 5 min to complete the quantitative detection of digoxin. The detection time is decreased 20–30 times in comparison to radioimmunoassay (RIA). The sensitivity to digoxin was about 2 ng/ml by naked eye, which is within the therapeutic and toxic ranges of digoxin. The results of serum samples obtained by IC strip were in agreement with those obtained by RIA. The IC strip was sufficiently sensitive and accurate to be used for the rapid detection of digoxin in serum samples.

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References

  1. Higashi, Y., Ikeda, Y., Yamamoto, R., et al. (2005). Life Sciences, 77, 1055–1067. doi:10.1016/j.lfs.2005.03.003.

    Article  CAS  Google Scholar 

  2. Ahmed, A., Young, J. B., & Gheorghiade, M. (2007). Canadian Medical Association Journal, 176, 641–643. doi:10.1503/cmaj.061239.

    Article  Google Scholar 

  3. Dasgupta, A., Kang, E., & Datta, P. (2006). Journal of Clinical Laboratory Analysis, 20, 204–208. doi:10.1002/jcla.20133.

    Article  CAS  Google Scholar 

  4. Al-musallam, M. (2006). [dissertation]. Molecular Medicine, Cranfield University, Cranfield Health.

  5. Graefe, K. A., Tang, Z., & Karnes, H. T. (2000). Journal of Chromatography. B, Biomedical Sciences and Applications, 45, 305–314. doi:10.1016/S0378-4347(00)00304-2.

    Article  Google Scholar 

  6. Ikeda, Y., & Fujii, Y. (2005). Biological & Pharmaceutical Bulletin, 28, 340–343. doi:10.1248/bpb.28.340.

    Article  CAS  Google Scholar 

  7. Dasgupta, A., Kang, E., Olsen, M., et al. (2007). Archives of Pathology & Laboratory Medicine, 131, 619–621.

    Google Scholar 

  8. Wu, Y. S., Lei, L. M., & Li, M. (2005). Di Yi Jun Yi Da Xue Xue Bao, 25, 761–765.

    CAS  Google Scholar 

  9. Paek, S. H., Lee, S. H., Cho, J. H., Kim, Y. S., et al. (2000). Methods (San Diego, CA), 22, 53–60. doi:10.1006/meth.2000.1036.

    CAS  Google Scholar 

  10. Xiulan, S., Xiaolian, Z., Jian, T., et al. (2005). International Journal of Food Microbiology, 99, 185–194. doi:10.1016/j.ijfoodmicro.2004.07.021.

    Article  Google Scholar 

  11. Lee, E. Y., Kang, J. H., Kim, K. A., et al. (2006). Journal of Immunological Methods, 308, 116–123. doi:10.1016/j.jim.2005.10.010.

    Article  CAS  Google Scholar 

  12. Molinelli, A., Grossalber, K., Führer, M., et al. (2008). Journal of Agricultural and Food Chemistry, 56, 2589–2594. doi:10.1021/jf800393j.

    Article  CAS  Google Scholar 

  13. Pastoor, R., Hatta, M., Theresia, H., et al. (2008). Diagnostic Microbiology and Infectious Disease, 61, 129–134. doi:10.1016/j.diagmicrobio.2007.12.014.

    Article  CAS  Google Scholar 

  14. Bienek, D. R., Biagini, R. E., Charlton, D. G., et al. (2008). Clinical and Vaccine Immunology; CVI, 15, 644–649. doi:10.1128/CVI.00473-07.

    Article  CAS  Google Scholar 

  15. Beck, O., Kraft, M., Moeller, M. R., et al. (2000). Annals of Clinical Biochemistry, 37(Pt 2), 199–204. doi:10.1258/0004563001899005.

    Article  CAS  Google Scholar 

  16. Chiao, D. J., Shyu, R. H., Hu, C. S., et al. (2004). Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 809, 37–41. doi:10.1016/j.jchromb.2004.05.033.

    Article  CAS  Google Scholar 

  17. Zhang, G., Wang, X., Zhi, A., et al. (2008). Food Additives and Contaminants, 25, 413–423. doi:10.1080/02652030701561452.

    Article  CAS  Google Scholar 

  18. Zhang, G. P., Wang, X. N., & Yang, J. F. (2006). Journal of Immunological Methods, 312, 27–33. doi:10.1016/j.jim.2006.02.017.

    Article  CAS  Google Scholar 

  19. Won-Bo, S., Yang, Z. Y., Kim, J. S., et al. (2007). Journal of Microbiology and Biotechnology, 17, 1629–1637.

    Google Scholar 

  20. Choi, M. J., Kim, S. Y., Choi, J., et al. (1999). Microchemical Journal, 63, 92–99. doi:10.1006/mchj.1999.1770.

    Article  CAS  Google Scholar 

  21. Gui, W. J., Wang, S. T., Guo, Y. R., & Zhu, G. N. (2008). Analytical Biochemistry, 377, 202–208. doi:10.1016/j.ab.2008.03.013.

    Article  CAS  Google Scholar 

  22. Hermanson, G. T., Mallia, A. K., & Smith, P. K. (1992). Immobilized affinity ligand techniques p. 54. San Diego: Academic.

    Google Scholar 

Download references

Acknowledgment

This research has been supported by the Endocrinology Metabolism Research Center of Tehran University of Medical Sciences, Tehran, I.R. Iran.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, P.O. Box 14395/1179, Tehran, Iran

    Kobra Omidfar, Afsaneh Besharatie & Susan Kashanian

  2. Department of Biology, School of Sciences, Razi University, Kermanshah, Iran

    Solmaz Kia

  3. Department of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscience & Nanotechnology Research Center (NNRC), Faculty of Science, Razi University, P.O. Box 67149, Kermanshah, Iran

    Soheila Kashanian

  4. Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Malihe Paknejad

  5. Endocrinology and Metabolism Research Center, Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

    Bagher Larijani

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  1. Kobra Omidfar
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  2. Solmaz Kia
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  3. Soheila Kashanian
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  5. Afsaneh Besharatie
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Correspondence to Kobra Omidfar or Soheila Kashanian.

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Omidfar, K., Kia, S., Kashanian, S. et al. Colloidal Nanogold-Based Immunochromatographic Strip Test for the Detection of Digoxin Toxicity. Appl Biochem Biotechnol 160, 843–855 (2010). https://doi.org/10.1007/s12010-009-8535-x

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  • DOI: https://doi.org/10.1007/s12010-009-8535-x

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