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Optimization of Effect Factors for Mycelial Growth and Exopolysaccharide Production by Schizophyllum commune

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This paper is concerned with the optimization of effect factors for mycelial growth and exopolysaccharide production by Schizophyllum commune by one-factor-at-a-time and orthogonal methods. The one-factor-at-a-time method was adopted to investigate the effects of six different compounds (sodium carboxymethylcellulose, l-glutamic acid, VB1, naphthalene acetic acid, oleic acid, and Tween 80) on mycelial growth and exopolysaccharide production. Among these factors, oleic acid, VB1 and Tween 80 were identified to be the most important factors. Subsequently, the concentration of oleic acid, VB1 and Tween 80 were optimized using the orthogonal matrix method. The effects of the factors on the mycelial growth of S. commune were in the order of oleic acid > VB1 > Tween 80, and those on exopolysaccharide production were in the same order. The optimal concentration for mycelia and exopolysaccharide were determined as oleic acid 0.1% (v/v), VB1 0.5 mg/L, and Tween 80 6 mg/L. The subsequent verification experiments confirmed the validity of the models. Under this optimized conditions in shake flask culture, the mycelial yield and exo-biopolymer production were 25.93 and 2.79 g/L, respectively, which were considerably higher than those obtained in the preliminary studies. The result was further confirmed in a 7-L fermentor experiments.

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References

  1. Watling, R., & Sweeney, J. (1972). Research Communications in Chemical Pathology and Pharmacology, 3, 214–226.

    CAS  Google Scholar 

  2. Desrochers, M., Jurasek, L., & Paice, M. G. (1981). Applied and Environmental Microbiology, 41, 222–228.

    CAS  Google Scholar 

  3. Leathers, T., Nunnally, M., & Price, N. (2006). Biotechnology Letters, 28, 623–626. doi:10.1007/s10529-006-0028-1.

    Article  CAS  Google Scholar 

  4. Rau, U. (1999). Production of schizophyllan. In C. Bucke (Ed.), Methods in biotechnology, Vol. 10. Carbohydrate biotechnology protocols. Totowa, NJ: Humana Press.

    Google Scholar 

  5. Hao, L.-M., Xing, X.-H., Zhang, Y.-K., Li, Z., Mu, J., & Wu, T.-Y. (2007). Food Science, 28, 320–323.

    CAS  Google Scholar 

  6. Schisler, L. C., & Volkoff, O. (1977). Mycologia, 69, 118–125. doi:10.2307/3758625.

    Article  Google Scholar 

  7. Survase, S. A., Saudagar, P. S., & Singhal, R. S. (2007). Bioresource Technology, 98, 1509–1512. doi:10.1016/j.biortech.2006.05.022.

    Article  CAS  Google Scholar 

  8. Yim, J. H., Kim, S. J., Ahn, S. H., & Lee, H. K. (2003). Biomolecular Engineering, 20, 273–280. doi:10.1016/S1389-0344(03)00070-4.

    Article  CAS  Google Scholar 

  9. Fukushima, Y., Itoh, H., Fukase, T., & Motai, H. (1991). Applied Microbiology and Biotechnology, 34, 586–590. doi:10.1007/BF00167904.

    Article  CAS  Google Scholar 

  10. Reese, E. T., & Maguire, A. (1969). Applied Microbiology, 17, 242–245.

    CAS  Google Scholar 

  11. Stasinopoulos, S. J., & Seviour, R. J. (1990). Biotechnology and Bioengineering, 36, 778–782.

    Article  CAS  Google Scholar 

  12. Yang, F. C., Ke, Y. F., & Kuo, S. S. (2000). Enzyme and Microbial Technology, 27, 295–301. doi:10.1016/S0141-0229(00)00213-1.

    Article  CAS  Google Scholar 

  13. DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Analytical Chemistry, 28, 350–356. doi:10.1021/ac60111a017.

    Article  CAS  Google Scholar 

  14. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428. doi:10.1021/ac60147a030.

    Article  CAS  Google Scholar 

  15. Malcolm, K. M. (1991). Biotechnology and Bioengineering, 38, 1261–1270. doi:10.1002/bit.260381102.

    Article  Google Scholar 

  16. Deckwer, W. D., & Schumpe, A. (1993). Chemical Engineering Science, 48, 889–911. doi:10.1016/0009-2509(93)80328-N.

    Article  CAS  Google Scholar 

  17. Schumpe, A., & Deckwer, W. D. (1982). Industrial & Engineering Chemistry Process Design and Development, 21, 706–711. doi:10.1021/i200019a028.

    Article  CAS  Google Scholar 

  18. Taga, N., Tanaka, K., & Ishizaki, A. (1997). Biotechnology and Bioengineering, 53, 529–533. doi:10.1002/(SICI)1097-0290(19970305)53:5<529::AID-BIT11>3.0.CO;2-B.

    Article  CAS  Google Scholar 

  19. Nampoothiri, K. M., Singhania, R. R., Sabarinath, C., & Pandey, A. (2003). Process Biochemistry, 38, 1513–1519. doi:10.1016/S0032-9592(02)00321-7.

    Article  CAS  Google Scholar 

  20. Survase, S. A., Saudagar, P. S., & Singhal, R. S. (2007). Bioresource Technology, 98, 410–415. doi:10.1016/j.biortech.2005.12.013.

    Article  CAS  Google Scholar 

  21. Stasinopoulos, S. J., & Seviour, R. J. (1990). Biotechnology and Bioengineering, 36, 778–782. doi:10.1002/bit.260360804.

    Article  CAS  Google Scholar 

  22. Zeng, G. M., Shi, J. G., Yuan, X. Z., Liu, J., Zhang, Z. B., Huang, G. H., et al. (2006). Enzyme and Microbial Technology, 39, 1451–1456. doi:10.1016/j.enzmictec.2006.03.035.

    Article  CAS  Google Scholar 

  23. Xu, C. P., Kim, S. W., Hwang, H. J., Choi, J. W., & Yun, J. W. (2003). Process Biochemistry, 38, 1025–1030. doi:10.1016/S0032-9592(02)00224-8.

    Article  CAS  Google Scholar 

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Acknowledgments

We thank Mrs. Guifang Deng for her kindly help in fermentation experiments.

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Authors and Affiliations

  1. Department of Chemical Engineering, Tsinghua University, Beijing, 100084, People’s Republic of China

    Li-min Hao & Xin-hui Xing

  2. Department of Food Research, The Quartermaster Equipment Institute of the General Logistics Department of PLA, Beijing, People’s Republic of China

    Li-min Hao

  3. Key Lab of Bioprocess of Beijing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Zheng Li

  4. Tianjin Polytechnic University, Tianjin, 300160, People’s Republic of China

    Zheng Li

  5. Research Center for China-hemp Materials, The Quartermaster Equipment Institute of the General Logistics Department of PLA, Beijing, People’s Republic of China

    Jian-chun Zhang

  6. Department of Biology, Hengshui College, Hebei, 053000, People’s Republic of China

    Jin-xu Sun

  7. Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science & Technology, Tianjin, 300222, People’s Republic of China

    Shi-ru Jia & Chang-sheng Qiao

  8. National Key Laboratory of High Altitude Medicine, Department of Hypoxic Physiology and Mountain Medicine, High Altitude Medical Research Institute, Xining, Qinghai, People’s Republic of China

    Tianyi Wu

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  1. Li-min Hao
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  2. Xin-hui Xing
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  4. Jian-chun Zhang
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Correspondence to Li-min Hao.

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Hao, Lm., Xing, Xh., Li, Z. et al. Optimization of Effect Factors for Mycelial Growth and Exopolysaccharide Production by Schizophyllum commune . Appl Biochem Biotechnol 160, 621–631 (2010). https://doi.org/10.1007/s12010-008-8507-6

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  • DOI: https://doi.org/10.1007/s12010-008-8507-6

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