Skip to main content
SpringerLink
Log in

Validation and Comparative Studies of Four Sulfonamide Immunoassays Based on the Same Generic Polyclonal Antibody

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

An Erratum to this article was published on 21 November 2009

An Erratum to this article was published on 21 November 2009

Abstract

Four different immunoassays based on the same generic polyclonal antibody were validated by high performance liquid chromatography, respectively. They also were compared with each other in terms of sensitivity, precision, and accuracy for the quantification or screening of sulfonamide residues in food samples. Correlation studies showed that there was a good correlation between the immunoassays and liquid chromatography data. The conventional plate assay has better precision and the plate-enhanced chemiluminescent assay has higher sensitivity. These two methods all could be used as quantification methods for large numbers of samples and complements of the conventional analytical methods in laboratory. The flow-through strip assay has higher sensitivity and the dip-stick strip assay was less affected by matrix effect. These two methods all could be used as valuable tools for the rapid on-site screening of sulfonamide residues in animal-derived food samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Franco, D. A., Webb, J., & Taylor, C. E. (1990). Journal of Food Protection, 53, 178–185.

    CAS  Google Scholar 

  2. Wang, S., Zhang, H. Y., Wang, L., Duan, Z. J., & Kennedy, I. R. (2006). Food Additives and Contaminants, 23, 362–384. doi:10.1080/02652030500499359.

    Article  CAS  Google Scholar 

  3. Sheth, H. B., & Sporns, P. (1991). Journal of Agricultural and Food Chemistry, 39, 1696–1700. doi:10.1021/jf00009a030.

    Article  CAS  Google Scholar 

  4. Korpimäki, T., Brockmann, E. V., Kuronen, O., Sarate, M., Lamminmäki, U., & Tuomola, M. (2004). Journal of Agricultural and Food Chemistry, 52, 40–47. doi:10.1021/jf034951i.

    Article  Google Scholar 

  5. Franek, M., Diblikova, I., Cernoch, I., Vass, M., & Hruska, K. (2006). Analytical Chemistry, 78, 1559–1567. doi:10.1021/ac0514422.

    Article  CAS  Google Scholar 

  6. Zhang, H. Y., Duan, Z. J., Wang, L., Zhang, Y., & Wang, S. (2006). Journal of Agricultural and Food Chemistry, 54, 4499–4505. doi:10.1021/jf060868u.

    Article  CAS  Google Scholar 

  7. Zhang, H. Y., Wang, L., Zhang, Y., Fang, G. Z., Zheng, W. J., & Wang, S. (2007). Journal of Agricultural and Food Chemistry, 55, 2079–2084. doi:10.1021/jf062896i.

    Article  CAS  Google Scholar 

  8. Hongyo, K., Itoh, Y., Hifumi, E., Takeyasu, A., & Uda, T. (1992). Journal of AOAC International, 75, 307–312.

    CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful for financial support from the Ministry of Science and Technology of the People’s Republic of China (project nos. 2006BAD05A06 and 2006AA10Z448) and the New Century Talent Program of Ministry of Education of the People’s Republic of China (project no. NECT-04-0243).

Author information

Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300222, People’s Republic of China

    Hongyan Zhang & Liguo Zang

  2. College of Life Science, Shandong Normal University, Jinan, 250014, People’s Republic of China

    Yan Zhang & Shuo Wang

Authors
  1. Hongyan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuo Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liguo Zang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shuo Wang.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s12010-009-8844-0

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, H., Zhang, Y., Wang, S. et al. Validation and Comparative Studies of Four Sulfonamide Immunoassays Based on the Same Generic Polyclonal Antibody. Appl Biochem Biotechnol 158, 642–652 (2009). https://doi.org/10.1007/s12010-008-8378-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-008-8378-x

Keywords

Search

Navigation