Abstract
Succinic acid is a useful chemical and its purification from fermentation broth by ion-exchange resins has widely drawn attention. In this study, pH neutralization in the process of adsorption of succinic acid from model solutions and fermentation broth by anion-exchange resin NERCB 04 has been tested. Adsorption capacity of NERCB 04 was about 0.41 g succinic acid/g resin at concentrations of succinic acid in the range of 10–50 g/L in packed column. In the process of succinic acid removal, pH of the system could also be neutralized. The neutralizing ability of the resin as a neutralizing agent has also been studied in the model cycle system and in the real fermentation cycle process. It was found that NERCB 04 showed stable adsorption capacity and pH neutralization ability after each regeneration. A certain amount of anion-exchange resin could neutralize the low pH values (pH 2–5) and maintain the system around pH 7.0. This means the anion-exchange resins have the function of neutralizing reagent in the process of adsorbing succinic acid.
Similar content being viewed by others
References
Werpy, T., & Petersen, G.(2004). Report no. TP51035523, US Department of Energy.
James, B., McKinlay, , Vieille, C., & Zeikus, J. G. (2007). Applied Microbiology and Biotechnology, 76, 727–740. doi:10.1007/s00253-007-1057-y.
Huh, Y. S., Jun, Y. S., Hong, Y. K., Song, H., Lee, S. Y., & Hong, W. H. (2006). Process Biochemistry, 41, 1461–1465. doi:10.1016/j.procbio.2006.01.020.
Tung, L. A., & King, C. J. (1994). Industrial & Engineering Chemistry Research, 33, 3217–3223. doi:10.1021/ie00036a041.
Berglund, K. A., Elankovan, P., & Glassner, D. A.(1991). US Patent no. 5034105.
Glassner, D. A., & Datta, R. US Patent no. 5143834.
Datta, R., & Glassner, D. A.(1992). US Patent no. 5168055.
Gluszcz, P., Jamroz, T., Sencio, B., & Ledakowicz, S. (2004). Bioprocess and Biosystems Engineering, 26, 185–190. doi:10.1007/s00449-003-0348-7.
Davison, B. H., Nghiem, N. P., & Richardson, G. L. (2004). Applied Biochemistry and Biotechnology, 12, 653–669. doi:10.1385/ABAB:114:1-3:653.
Van der Werf, M. J., Guettler, M. V., Jain, M. K., & Zeikus, J. G. (1997). Archives of Microbiology, 167, 332–342. doi:10.1007/s002030050452.
Han, R. P., Wang, Y., Zou, W. H., Wang, Y. F., & Shi, (2007). Journal of Hazardous Materials, 145, 331–335. doi:10.1016/j.jhazmat.2006.12.027.
Vijayaraghavan, K., & Prabu, D. (2006). Journal of Hazardous Materials, 137, 558–564. doi:10.1016/j.jhazmat.2006.02.030.
Guettler, M. V., RumLer, D., & Jain, M. K. (1999). International Journal of Systematic Bacteriology, 49, 207–216.
Sa’áncheza, A. M., Bennett, G. N., & San, K. Y. (2006). Metabolic Engineering, 8, 209–226. doi:10.1016/j.ymben.2005.11.004.
McKinlay, J. B., Zeikus, J. G., & Vieille, C. (2005). Applied and Environmental Microbiology, 71, 6651–6656. doi:10.1128/AEM.71.11.6651-6656.2005.
Acknowledgement
This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences, grant no. KSCX2-YW-G-021.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, Q., Li, Wl., Wang, D. et al. pH Neutralization while Succinic Acid Adsorption onto Anion-Exchange Resins. Appl Biochem Biotechnol 160, 438–445 (2010). https://doi.org/10.1007/s12010-008-8355-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-008-8355-4